NF ISO 21528-1

NF ISO 21528-1

December 2004
Standard Cancelled

Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 1 : detection and enumeration by MPN technique with pre-enrichment

ISO 21528-1:2004 specifies a method, with pre-enrichment, for the detection of Enterobacteriaceae. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling. Enumeration is carried out by calculation of the most probable number (MPN) after incubation at 37 °C in liquid medium. This method is applicable when the microorganisms sought are expected to need resuscitation before enrichment, and when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample. A limitation on the applicability of ISO 21528-1:2004 is imposed by the susceptibility of the method to a large degree of variability

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Main informations

Collections

National standards and national normative documents

Publication date

December 2004

Number of pages

20 p.

Reference

NF ISO 21528-1

ICS Codes

07.100.30   Food microbiology

Classification index

V08-039-1

Print number

1 - 21/12/2004

International kinship

ISO 21528-1:2004
Sumary
Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 1 : detection and enumeration by MPN technique with pre-enrichment

ISO 21528-1:2004 specifies a method, with pre-enrichment, for the detection of Enterobacteriaceae. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.

Enumeration is carried out by calculation of the most probable number (MPN) after incubation at 37 °C in liquid medium.

This method is applicable when the microorganisms sought are expected to need resuscitation before enrichment, and when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.

A limitation on the applicability of ISO 21528-1:2004 is imposed by the susceptibility of the method to a large degree of variability

Replaced standards (2)
NF ISO 7402
December 1993
Standard Cancelled
Microbiology. General guidance for the enumeration of {Enterobacteriaceae} without resuscitation. Mpn technique and colony count technique.

NF ISO 8523
February 1992
Standard Cancelled
Microbiology. General guidance for the detection of {Enterobacteriaceae} with pre-enrichment.

Standard replaced by (1)
NF EN ISO 21528-1
July 2017
Standard Current
Microbiology of the food chain - Horizontal method for the detection and enumeration of Enterobacteriaceae - Part 1 : Detection of Enterobacteriaceae

<p>ISO 21528-1:2017 specifies a method, with enrichment, for the detection of <i>Enterobacteriaceae</i>. It is applicable to</p> <p>- products intended for human consumption and the feeding of animals, and</p> <p>- environmental samples in the area of primary production, food production and food handling.</p> <p>This method is applicable</p> <p>- when the microorganisms sought are expected to need resuscitation by enrichment, and</p> <p>- when the number sought is expected to be below 100 per millilitre or per gram of test sample.</p> <p>A limitation on the applicability of ISO 21528-1:2017 is imposed by the susceptibility of the method to a large degree of variability (see Clause 11).</p> <p>NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See Annex A.</p>

Table of contents
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  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    2
  • 4 Principe
    2
  • 4.1 Recherche des Enterobacteriaceae (voir l'Annexe B)
    2
  • 4.2 Dénombrement par la technique NPP (voir l'Annexe B)
    3
  • 5 Diluants, milieux de culture et réactifs
    3
  • 6 Appareillage et verrerie
    7
  • 7 Échantillonnage
    7
  • 8 Préparation de l'échantillon soumis à l'essai
    7
  • 9 Mode opératoire
    8
  • 9.1 Généralités
    8
  • 9.2 Méthode de détection
    8
  • 9.3 Méthode de dénombrement (méthode NPP)
    8
  • 9.4 Isolement et identification pour confirmation
    9
  • 9.5 Repiquage des colonies sélectionnées
    9
  • 9.6 Essais de confirmation biochimique
    9
  • 10 Expression des résultats
    10
  • 10.1 Tubes confirmés comme positifs
    10
  • 10.2 Méthode de recherche
    10
  • 10.3 Méthode de dénombrement: calcul du nombre le plus probable (NPP)
    10
  • 11 Précision
    10
  • 12 Rapport d'essai
    10
  • Annexe A (normative) Diagramme pour la mise en oeuvre de la méthode de recherche
    11
  • Annexe B (normative) Diagramme pour la mise en oeuvre de la technique NPP
    12
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