ISO 20837:2006

Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for sample preparation for qualitative detection

ISO 20837:2006 provides criteria and examples for sample preparation in order to obtain PCR-compatible samples or nucleic acids of suitable quality and quantity for PCR.

It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B.

ISO 20837:2006 has been established for food matrices, but could also be applied to feed and agricultural/environmental matrices with some adaptations, if necessary.

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