NF EN ISO 15753

NF EN ISO 15753

November 2006
Standard Cancelled

Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons

ISO 15753:2006 describes two methods for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in animal and vegetable fats and oils: a general method, and a method specific for coconut oil and short-chain vegetable oils. These methods are not quantitative for the very volatile compounds such as naphthalene, acenaphthene and fluorene. Due to interferences provided by the matrix itself, palm oil and olive pomace oil cannot be analysed using this method.

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Main informations

Collections

National standards and national normative documents

Publication date

November 2006

Number of pages

29 p.

Reference

NF EN ISO 15753

ICS Codes

67.200.10   Animal and vegetable fats and oils

Classification index

T60-267

Print number

1 - 14/11/2006

International kinship

European kinship

EN ISO 15753:2006
Sumary
Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons

ISO 15753:2006 describes two methods for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in animal and vegetable fats and oils: a general method, and a method specific for coconut oil and short-chain vegetable oils.

These methods are not quantitative for the very volatile compounds such as naphthalene, acenaphthene and fluorene. Due to interferences provided by the matrix itself, palm oil and olive pomace oil cannot be analysed using this method.

Standard replaced by (1)
NF EN ISO 15753
June 2016
Standard Current
Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons

<p>ISO 15753:2016 describes two methods for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in animal and vegetable fats and oils:</p> <p>- a general method;</p> <p>- a specific method for coconut oil and vegetable oils with short-chain fatty acids.</p> <p>These methods are not quantitative for the very volatile compounds such as naphthalene, acenaphthene and fluorene. Due to interferences provided by the matrix itself, palm oil and olive pomace oil cannot be analysed using this method.</p> <p>The quantification limit is 0,2 µg/kg for almost all compounds analysed, except for fluoranthene and benzo(g,h,i)perylene, where the quantification limit is 0,3 µg/kg, and indeno(1,2,3-c,d)pyrene, where the quantification limit is 1,0 µg/kg.</p> <p>NOTE The results for olive pomace oil in Annex B show that this method is not applicable to this type of oil. The precision data determined are very poor. </p> <p>Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this International Standard.</p>

Table of contents
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  • Avant-propos
    iv
  • 1
    Domaine d'application 1
  • 2
    Références normatives 1
  • 3
    Termes et définitions 1
  • 4
    Principe 2
  • 5
    Réactifs et matériaux 2
  • 6
    Appareillage 3
  • 7
    Échantillonnage 5
  • 8
    Préparation de l'échantillon 5
  • 9
    Mode opératoire pour la détermination des HAP à partir des corps gras: Méthode générale 5
  • 9.1
    Remarques préliminaires 5
  • 9.2
    Essai à blanc 5
  • 9.3
    Détermination des valeurs des taux de récupération (sans matrice) 6
  • 9.4
    Extraction (extraction liquide/liquide) 6
  • 9.5
    Purification sur des cartouches de phase greffée en C18 (extraction solide/liquide) 6
  • 9.6
    Purification sur des cartouches de phase greffée de Florisil (extraction solide/liquide) 7
  • 10
    Mode opératoire pour la détermination des HAP à partir des corps gras: Méthode spécifique pour l'huile de coprah 7
  • 10.1
    Première extraction (extraction liquide/liquide) 7
  • 10.2
    Deuxième extraction (extraction liquide/liquide) 8
  • 10.3
    Purification sur des cartouches de phase greffée en C18 (extraction solide/liquide) 8
  • 10.4
    Purification sur des cartouches de phase greffée de Florisil (extraction solide/liquide) 9
  • 11
    Chromatographie liquide à haute performance (CLHP) 9
  • 11.1
    Conditions de fonctionnement 9
  • 11.2
    Paramètres de détection 10
  • 11.3
    Analyse des échantillons et des étalons 12
  • 11.4
    Confirmation de la présence des HAP 13
  • 12
    Expression des résultats 13
  • 13
    Fidélité 13
  • 13.1
    Essai interlaboratoires 13
  • 13.2
    Répétabilité 14
  • 13.3
    Reproductibilité 14
  • 14
    Rapport d'essai 14
  • Annexe A (informative) Valeurs des taux de récupération, schémas fonctionnels, chromatogrammes et séquences d'injection
    15
  • Annexe B (informative) Résultats de l'essai interlaboratoires
    20
  • Bibliographie
    23
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