NF EN ISO 16050

NF EN ISO 16050

October 2011
Standard Current

Foodstuffs - Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived products - High-performance liquid chromatographic method

ISO 16050:2003 specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B1, and for the sum of aflatoxins B1, B2, G1 and G2, is 8 micrograms per kilogram. The method has been validated for maize containing 24,5 micrograms per kilogram, for peanut butter containing 8,4 micrograms per kilogram, and for raw peanuts containing 16 micrograms per kilogram of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.

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Main informations

Collections

National standards and national normative documents

Publication date

October 2011

Number of pages

21 p.

Reference

NF EN ISO 16050

ICS Codes

67.060   Cereals, pulses and derived products
67.080.10   Fruits and derived products
67.200.20   Oilseeds

Classification index

V03-122

Print number

1 - 03/10/2011

International kinship

ISO 16050:2003

European kinship

EN ISO 16050:2011
Sumary
Foodstuffs - Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived products - High-performance liquid chromatographic method

ISO 16050:2003 specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B1, and for the sum of aflatoxins B1, B2, G1 and G2, is 8 micrograms per kilogram.

The method has been validated for maize containing 24,5 micrograms per kilogram, for peanut butter containing 8,4 micrograms per kilogram, and for raw peanuts containing 16 micrograms per kilogram of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.

Replaced standards (1)
NF EN 12955
October 1999
Standard Cancelled
Foodstuffs - Determination of aflatoxin b1 , and the sum of aflatoxins B1, B2, G1 and G2 in cereals, shell-fruits and derived products - High performance liquid chromatographic method with post column derivatization and immunoaffinity column clean up

Le présent document spécifie une méthode de détermination des teneurs en aflatoxi-nes supérieures à 8 microgrammes/kg dans des aliments tels que maïs, beurre d'arachide,céréales, produits oléagineux, fruits secs,...

Table of contents
View the extract
  • Avant-propos
    iv
  • 1
    Domaine d'application 1
  • 2
    Références normatives 1
  • 3
    Principe 1
  • 4
    Réactifs 1
  • 5
    Appareillage 4
  • 6
    Mode opératoire 5
  • 6.1
    Généralités 5
  • 6.2
    Extraction 6
  • 6.3
    Purification 6
  • 6.4
    Conditions de fonctionnement de la CLHP 6
  • 6.5
    Identification 7
  • 6.6
    Courbe d'étalonnage 7
  • 6.7
    Détermination 7
  • 7
    Calcul des résultats 7
  • 8
    Fidélité 8
  • 8.1
    Essai interlaboratoires 8
  • 8.2
    Répétabilité 8
  • 8.3
    Reproductibilité 9
  • 9
    Rapport d'essai 10
  • Annexe A (informative) Résultats de l'essai interlaboratoires
    11
  • Bibliographie
    13
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