NF EN ISO 16050
Foodstuffs - Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived products - High-performance liquid chromatographic method
ISO 16050:2003 specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B1, and for the sum of aflatoxins B1, B2, G1 and G2, is 8 micrograms per kilogram. The method has been validated for maize containing 24,5 micrograms per kilogram, for peanut butter containing 8,4 micrograms per kilogram, and for raw peanuts containing 16 micrograms per kilogram of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.
ISO 16050:2003 specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B1, and for the sum of aflatoxins B1, B2, G1 and G2, is 8 micrograms per kilogram.
The method has been validated for maize containing 24,5 micrograms per kilogram, for peanut butter containing 8,4 micrograms per kilogram, and for raw peanuts containing 16 micrograms per kilogram of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.
Le présent document spécifie une méthode de détermination des teneurs en aflatoxi-nes supérieures à 8 microgrammes/kg dans des aliments tels que maïs, beurre d'arachide,céréales, produits oléagineux, fruits secs,...
- Avant-proposiv
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1Domaine d'application 1
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2Références normatives 1
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3Principe 1
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4Réactifs 1
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5Appareillage 4
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6Mode opératoire 5
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6.1Généralités 5
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6.2Extraction 6
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6.3Purification 6
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6.4Conditions de fonctionnement de la CLHP 6
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6.5Identification 7
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6.6Courbe d'étalonnage 7
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6.7Détermination 7
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7Calcul des résultats 7
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8Fidélité 8
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8.1Essai interlaboratoires 8
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8.2Répétabilité 8
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8.3Reproductibilité 9
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9Rapport d'essai 10
- Annexe A (informative) Résultats de l'essai interlaboratoires11
- Bibliographie13
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