NF EN ISO 16212

NF EN ISO 16212

July 2017
Standard Current

Cosmetics - Microbiology - Enumeration of yeast and mould

ISO 16212:2017 gives general guidelines for enumeration of yeast and mould present in cosmetics by counting the colonies on selective agar medium after aerobic incubation. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which ISO 16212:2017 is applicable. Products considered to present a low microbiological risk (see ISO 29621) include those with low water activity or extreme pH values, hydro-alcoholic products, etc. Because of the large variety of cosmetic products within this field of application, this method might not be suited to some products in every detail (e.g. certain water-immiscible products). Other methods (e.g. automated) can be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable. Yeast enumerated can be identified using suitable identification tests, for example, tests described in the standards listed in the Bibliography. Mould enumerated can be identified by other appropriate methods, if necessary.

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Main informations

Collections

National standards and national normative documents

Publication date

July 2017

Number of pages

29 p.

Reference

NF EN ISO 16212

ICS Codes

07.100.40   Cosmetics microbiology

Classification index

T75-608

Print number

1

International kinship

European kinship

EN ISO 16212:2017
Sumary
Cosmetics - Microbiology - Enumeration of yeast and mould

ISO 16212:2017 gives general guidelines for enumeration of yeast and mould present in cosmetics by counting the colonies on selective agar medium after aerobic incubation.

In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which ISO 16212:2017 is applicable. Products considered to present a low microbiological risk (see ISO 29621) include those with low water activity or extreme pH values, hydro-alcoholic products, etc.

Because of the large variety of cosmetic products within this field of application, this method might not be suited to some products in every detail (e.g. certain water-immiscible products). Other methods (e.g. automated) can be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.

Yeast enumerated can be identified using suitable identification tests, for example, tests described in the standards listed in the Bibliography. Mould enumerated can be identified by other appropriate methods, if necessary.

Replaced standards (1)
NF EN ISO 16212
August 2011
Standard Cancelled
Cosmetics - Microbiology - Enumeration of yeast and mould

<p>ISO 16212:2008 gives general guidelines for enumeration of yeast and mould present in cosmetics, by counting the colonies on selective agar medium after aerobic incubation.</p> <p>In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis, so as to determine the types of cosmetic products to which ISO 16212:2008 is applicable. Products considered to present a low microbiological risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc.</p>

Table of contents
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  • Avant-propos
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principes
  • 5 Diluants, agents neutralisants et milieux de culture
  • 6 Matériel et verrerie
  • 7 Souches de micro-organismes
  • 8 Manipulation des produits cosmétiques et des échantillons de laboratoire
  • 9 Mode opératoire
  • 10 Dénombrement des colonies (méthodes de dénombrement sur gélose en boîtes de Petri et par filtration sur membrane)
  • 11 Expression des résultats
  • 12 Neutralisation des propriétés fongicides du produit
  • 13 Rapport d'essai
  • Annexe A (informative) Autres diluants neutralisants
  • Annexe B (informative) Autres diluants
  • Annexe C (informative) Autres milieux de culture
  • Annexe D (informative) Neutralisants de l'activité fongicide des conservateurset des liquides de rinçage
  • Bibliographie
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