NF EN ISO 16654

July 2001

Standard Current

Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157

La présente Norme internationale spécifie une méthode horizontale pour la recherche des Escherichia coli sérogroupe O157. Soumise aux restrictions énoncées dans l'introduction, la présente Norme internationale est applicable aux produits destinés à la consommation humaine ou aux produits alimentaires.

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Main informations

Collections

National standards and national normative documents

Publication date

July 2001

Number of pages

25 p.

Reference

NF EN ISO 16654

ICS Codes

07.100.30 Food microbiology

Classification index

V08-032

Print number

1 - 19/09/2003

International kinship

ISO 16654:2001

European kinship

EN ISO 16654:2001

Sumary

Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157

Table of contents

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Avant-propos
iv
Introduction
v
1 Domaine d'application
1
2 Références normatives
1
3 Terme et définition
1
4 Principe
2
5 Milieux de culture, réactifs et antisérum
2
6 Appareillage et verrerie
8
7 Échantillonnage
9
8 Préparation de l'échantillon pour essai
9
9 Mode opératoire (voir l'annexe A)
9
9.1 Prise d'essai et suspension mère
9
9.2 Enrichissement
9
9.3 Séparation immunomagnétique (IMS)
9
9.4 Ensemencement sur les géloses sélectives et identification des colonies d'E. coll O157
10
9.5 Confirmation
11
9.6 Identification complémentaire
12
10 Assurance qualité
12
10.1 Souches de contrôle pour l'assurance qualité
12
10.2 Méthode de culture
12
11 Expression des résultats
12
12 Rapport d'essai
12
Annexe A (normative) Diagramme du mode opératoire
13
Bibliographie
14

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