NF EN ISO 20837

NF EN ISO 20837

July 2006
Standard Current

Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for sample preparation for qualitative detection

ISO 20837:2006 provides criteria and examples for sample preparation in order to obtain PCR-compatible samples or nucleic acids of suitable quality and quantity for PCR. It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B. ISO 20837:2006 has been established for food matrices, but could also be applied to feed and agricultural/environmental matrices with some adaptations, if necessary.

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Main informations

Collections

National standards and national normative documents

Publication date

July 2006

Number of pages

18 p.

Reference

NF EN ISO 20837

ICS Codes

07.100.30   Food microbiology

Classification index

V03-029

Print number

1 - 29/06/2006

International kinship

ISO 20837:2006

European kinship

EN ISO 20837:2006
Sumary
Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for sample preparation for qualitative detection

ISO 20837:2006 provides criteria and examples for sample preparation in order to obtain PCR-compatible samples or nucleic acids of suitable quality and quantity for PCR.

It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B.

ISO 20837:2006 has been established for food matrices, but could also be applied to feed and agricultural/environmental matrices with some adaptations, if necessary.

Table of contents
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  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Principe
    1
  • 4 Exigences générales de laboratoire
    2
  • 5 Réactifs, appareillage et matériel
    2
  • 6 Mode opératoire
    2
  • Annexe A (informative) Normes relatives à l'enrichissement des micro-organismes (bactéries)
    5
  • Annexe B (informative) Méthode pour l'extraction d'ADN à partir de bactéries Gram-négatives
    6
  • Bibliographie
    8
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