NF EN ISO 21569

April 2006

Standard Current

Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods

ISO 21569:2005 describes the procedure to qualitatively detect genetically modified organisms (GMOs) and derived products by analysing the nucleic acids extracted from the sample under study. The main focus is on polymerase chain reaction (PCR) based amplification methods. It gives general requirements for the specific detection and identification of target nucleic acid sequences (DNA) and for the confirmation of the identity of the amplified DNA sequence. Guidelines, minimum requirements and performance criteria laid down in ISO 21569:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories. ISO 21569:2005 has been established for food matrices, but could also be applied to other matrices (e.g. feed and plant samples from the environment). Specific examples of methods are provided in Annexes A to D.

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Main informations

Collections

National standards and national normative documents

Publication date

April 2006

Number of pages

81 p.

Reference

NF EN ISO 21569

ICS Codes

07.080 Biology. Botany. Zoology

67.050 General methods of tests and analysis for food products

Classification index

V03-022

Print number

1 - 20/03/2006

International kinship

ISO 21569:2005

European kinship

EN ISO 21569:2005

Sumary

Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods

It gives general requirements for the specific detection and identification of target nucleic acid sequences (DNA) and for the confirmation of the identity of the amplified DNA sequence.

Guidelines, minimum requirements and performance criteria laid down in ISO 21569:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories.

ISO 21569:2005 has been established for food matrices, but could also be applied to other matrices (e.g. feed and plant samples from the environment).

Specific examples of methods are provided in Annexes A to D.

Replaced standards (1)

XP V03-020-1

September 2003

Standard Cancelled

Foodstuffs - Detection and quantification of genetically modified vegetal organisms and derived products - Part 1 : guidelines and requirements for methods based on the detection of nucleic acids

Le présent document a pour objectif d'homogénéiser les résultats obtenus dans les laboratoires par la définition d'un cadre général auquel doit satisfaire toute méthode de détection des organismes végétaux génétiquement modifiés (OGM) basée sur les acides nucléiques et plus particulièrement sur la technique PCR (réaction de polymérisation en chaîne).

Table of contents

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Avant-propos
iv
Introduction
v
1 Domaine d'application
1
2 Références normatives
1
3 Termes et définitions
1
4 Principe de la méthode
2
5 Réactifs
2
6 Appareillage et équipements
3
7 Mode opératoire
3
8 Interprétation
6
9 Expression des résultats et assurance qualité
6
10 Rapport d'essai
7
Annexe A (informative) Méthodes spécifiques au taxon
8
Annexe B (informative) Méthodes de criblage
25
Annexe C (informative) Méthodes spécifiques au construit
41
Annexe D (informative) Méthodes spécifiques aux évènements
64
Bibliographie
69

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