NF EN ISO 21570
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods
ISO 21570:2005 provides the overall framework of quantitative methods for the detection of genetically modified organisms (GMO) in foodstuffs, using the polymerase chain reaction (PCR). It defines general requirements for the specific amplification of DNA target sequences in order to quantify the relative GMO-derived DNA content and to confirm the identity of the amplified DNA sequence. Guidelines, minimum requirements and performance criteria laid down in ISO 21570:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories. ISO 21570:2005 has been established for food matrices, but is also applicable to other matrices, e.g. feed and plant samples from the environment.
ISO 21570:2005 provides the overall framework of quantitative methods for the detection of genetically modified organisms (GMO) in foodstuffs, using the polymerase chain reaction (PCR).
It defines general requirements for the specific amplification of DNA target sequences in order to quantify the relative GMO-derived DNA content and to confirm the identity of the amplified DNA sequence.
Guidelines, minimum requirements and performance criteria laid down in ISO 21570:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories.
ISO 21570:2005 has been established for food matrices, but is also applicable to other matrices, e.g. feed and plant samples from the environment.
Le présent document a pour objectif d'homogénéiser les résultats obtenus dans les laboratoires par la définition d'un cadre général auquel doit satisfaire toute méthode de détection des organismes végétaux génétiquement modifiés (OGM) basée sur les acides nucléiques et plus particulièrement sur la technique PCR (réaction de polymérisation en chaîne).
- Avant-proposv
- Introductionvi
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1 Domaine d'application1
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2 Références normatives1
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3 Termes et définitions1
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4 Principe2
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4.1 Généralités2
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4.2 Amplification, détection et confirmation des produits PCR2
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4.3 Quantification des produits PCR2
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5 Réactifs2
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6 Appareillage et équipements2
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7 Lignes directrices relatives au mode opératoire3
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7.1 Généralités3
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7.2 Stabilité de la séquence cible3
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7.3 Étalonnage de l'analyse3
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7.4 Considérations relatives à la quantification3
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7.5 Exigences relatives à l'assurance qualité4
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8 Interprétation4
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9 Expression des résultats5
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10 Rapport d'essai5
- Annexe A (informative) Méthodes spécifiques du taxon cible6
- Annexe B (informative) Méthodes de criblage13
- Annexe C (informative) Méthodes construit-spécifiques du gène21
- Annexe D (informative) Méthodes événement-spécifiques93
- Bibliographie107
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