NF EN ISO 21570

NF EN ISO 21570

June 2006
Standard Current

Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods

ISO 21570:2005 provides the overall framework of quantitative methods for the detection of genetically modified organisms (GMO) in foodstuffs, using the polymerase chain reaction (PCR). It defines general requirements for the specific amplification of DNA target sequences in order to quantify the relative GMO-derived DNA content and to confirm the identity of the amplified DNA sequence. Guidelines, minimum requirements and performance criteria laid down in ISO 21570:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories. ISO 21570:2005 has been established for food matrices, but is also applicable to other matrices, e.g. feed and plant samples from the environment.

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Main informations

Collections

National standards and national normative documents

Publication date

June 2006

Number of pages

123 p.

Reference

NF EN ISO 21570

ICS Codes

07.080   Biology. Botany. Zoology
67.050   General methods of tests and analysis for food products

Classification index

V03-025

Print number

2 - 01/03/2007

International kinship

ISO 21570:2005

European kinship

EN ISO 21570:2005
Sumary
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods

ISO 21570:2005 provides the overall framework of quantitative methods for the detection of genetically modified organisms (GMO) in foodstuffs, using the polymerase chain reaction (PCR).

It defines general requirements for the specific amplification of DNA target sequences in order to quantify the relative GMO-derived DNA content and to confirm the identity of the amplified DNA sequence.

Guidelines, minimum requirements and performance criteria laid down in ISO 21570:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories.

ISO 21570:2005 has been established for food matrices, but is also applicable to other matrices, e.g. feed and plant samples from the environment.

Replaced standards (1)
XP V03-020-1
September 2003
Standard Cancelled
Foodstuffs - Detection and quantification of genetically modified vegetal organisms and derived products - Part 1 : guidelines and requirements for methods based on the detection of nucleic acids

Le présent document a pour objectif d'homogénéiser les résultats obtenus dans les laboratoires par la définition d'un cadre général auquel doit satisfaire toute méthode de détection des organismes végétaux génétiquement modifiés (OGM) basée sur les acides nucléiques et plus particulièrement sur la technique PCR (réaction de polymérisation en chaîne).

Table of contents
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  • Avant-propos
    v
  • Introduction
    vi
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    1
  • 4 Principe
    2
  • 4.1 Généralités
    2
  • 4.2 Amplification, détection et confirmation des produits PCR
    2
  • 4.3 Quantification des produits PCR
    2
  • 5 Réactifs
    2
  • 6 Appareillage et équipements
    2
  • 7 Lignes directrices relatives au mode opératoire
    3
  • 7.1 Généralités
    3
  • 7.2 Stabilité de la séquence cible
    3
  • 7.3 Étalonnage de l'analyse
    3
  • 7.4 Considérations relatives à la quantification
    3
  • 7.5 Exigences relatives à l'assurance qualité
    4
  • 8 Interprétation
    4
  • 9 Expression des résultats
    5
  • 10 Rapport d'essai
    5
  • Annexe A (informative) Méthodes spécifiques du taxon cible
    6
  • Annexe B (informative) Méthodes de criblage
    13
  • Annexe C (informative) Méthodes construit-spécifiques du gène
    21
  • Annexe D (informative) Méthodes événement-spécifiques
    93
  • Bibliographie
    107
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