NF EN ISO 21571
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Nucleic acid extraction
ISO 21571:2005 provides general requirements and specific methods for DNA extraction/purification and quantification. These methods are described in Annexes A and B. ISO 21571:2005 has been established for food matrices, but could also be applicable to other matrices, such as grains and feed. It has been designed as an integral part of nucleic-acid-based analytical methods, in particular ISO 21569 on qualitative analytical methods, and ISO 21570 on quantitative analytical methods.
ISO 21571:2005 provides general requirements and specific methods for DNA extraction/purification and quantification. These methods are described in Annexes A and B.
ISO 21571:2005 has been established for food matrices, but could also be applicable to other matrices, such as grains and feed.
It has been designed as an integral part of nucleic-acid-based analytical methods, in particular ISO 21569 on qualitative analytical methods, and ISO 21570 on quantitative analytical methods.
Le présent document a pour objectif d'homogénéiser les résultats obtenus dans les laboratoires par la définition d'un cadre général auquel doit satisfaire toute méthode de détection des organismes végétaux génétiquement modifiés (OGM) basée sur les acides nucléiques et plus particulièrement sur la technique PCR (réaction de polymérisation en chaîne).
- Avant-proposiv
- Introductionv
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1 Domaine d'application1
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2 Références normatives1
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3 Principe1
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3.1 Généralités1
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3.2 Extraction de l'ADN2
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3.3 Quantification de l'ADN2
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4 Exigences générales applicables aux laboratoires2
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5 Mode opératoire2
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5.1 Préparation de la prise d'essai2
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5.2 Extraction/purification de l'ADN4
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5.3 Quantification de l'ADN extrait5
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5.4 Stabilité de l'ADN extrait6
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6 Interprétation6
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7 Rapport d'essai6
- Annexe A (informative) Méthodes pour l'extraction de l'ADN8
- Annexe B (informative) Méthodes de quantification de l'ADN extrait36
- Bibliographie43
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