NF EN ISO 21571

NF EN ISO 21571

August 2005
Standard Current

Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Nucleic acid extraction

ISO 21571:2005 provides general requirements and specific methods for DNA extraction/purification and quantification. These methods are described in Annexes A and B. ISO 21571:2005 has been established for food matrices, but could also be applicable to other matrices, such as grains and feed. It has been designed as an integral part of nucleic-acid-based analytical methods, in particular ISO 21569 on qualitative analytical methods, and ISO 21570 on quantitative analytical methods.

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Main informations

Collections

National standards and national normative documents

Publication date

August 2005

Number of pages

53 p.

Reference

NF EN ISO 21571

ICS Codes

07.080   Biology. Botany. Zoology
67.050   General methods of tests and analysis for food products

Classification index

V03-023

Print number

2 - 01/06/2006

International kinship

ISO 21571:2005

European kinship

EN ISO 21571:2005
Sumary
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Nucleic acid extraction

ISO 21571:2005 provides general requirements and specific methods for DNA extraction/purification and quantification. These methods are described in Annexes A and B.

ISO 21571:2005 has been established for food matrices, but could also be applicable to other matrices, such as grains and feed.

It has been designed as an integral part of nucleic-acid-based analytical methods, in particular ISO 21569 on qualitative analytical methods, and ISO 21570 on quantitative analytical methods.

Replaced standards (1)
XP V03-020-1
September 2003
Standard Cancelled
Foodstuffs - Detection and quantification of genetically modified vegetal organisms and derived products - Part 1 : guidelines and requirements for methods based on the detection of nucleic acids

Le présent document a pour objectif d'homogénéiser les résultats obtenus dans les laboratoires par la définition d'un cadre général auquel doit satisfaire toute méthode de détection des organismes végétaux génétiquement modifiés (OGM) basée sur les acides nucléiques et plus particulièrement sur la technique PCR (réaction de polymérisation en chaîne).

Table of contents
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  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Principe
    1
  • 3.1 Généralités
    1
  • 3.2 Extraction de l'ADN
    2
  • 3.3 Quantification de l'ADN
    2
  • 4 Exigences générales applicables aux laboratoires
    2
  • 5 Mode opératoire
    2
  • 5.1 Préparation de la prise d'essai
    2
  • 5.2 Extraction/purification de l'ADN
    4
  • 5.3 Quantification de l'ADN extrait
    5
  • 5.4 Stabilité de l'ADN extrait
    6
  • 6 Interprétation
    6
  • 7 Rapport d'essai
    6
  • Annexe A (informative) Méthodes pour l'extraction de l'ADN
    8
  • Annexe B (informative) Méthodes de quantification de l'ADN extrait
    36
  • Bibliographie
    43
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