NF EN ISO 22174

June 2005

Standard Current

Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions

ISO 22174:2004 gives the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). It is applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne pathogens using the polymerase chain reaction (PCR) The minimum requirements laid down in ISO 22174:2004 are intended to ensure that comparable and reproducible results are obtained in different laboratories. ISO 22174:2004 has been established for food-borne pathogens in or isolated from food and feed matrices, but is also applicable to other matrices (e.g. environmental samples) and for the detection of non-pathogenic microorganisms.

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Main informations

Collections

National standards and national normative documents

Publication date

June 2005

Number of pages

22 p.

Reference

NF EN ISO 22174

ICS Codes

07.100.30 Food microbiology

Classification index

V08-410

Print number

1 - 06/06/2005

International kinship

ISO 22174:2005

European kinship

EN ISO 22174:2005

Sumary

Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions

ISO 22174:2004 gives the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). It is applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne pathogens using the polymerase chain reaction (PCR)

The minimum requirements laid down in ISO 22174:2004 are intended to ensure that comparable and reproducible results are obtained in different laboratories.

ISO 22174:2004 has been established for food-borne pathogens in or isolated from food and feed matrices, but is also applicable to other matrices (e.g. environmental samples) and for the detection of non-pathogenic microorganisms.

Table of contents

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Avant-propos
iv
Introduction
v
1 Domaine d'application
1
2 Références normatives
1
3 Termes et définitions
2
4 Principe
6
4.1 Généralités
6
4.2 Enrichissement microbien préliminaire
7
4.3 Préparation de l'acide nucléique
7
4.4 Amplification par PCR
7
4.5 Détection et confirmation des produits PCR
7
5 Matériau d'essai
7
6 Exigences générales de laboratoire
8
6.1 Généralités
8
6.2 Personnel
8
6.3 Équipement du laboratoire
8
6.4 Gestion des déchets
9
7 Réactifs
9
8 Appareillage et équipement
9
8.1 Généralités
9
8.2 Considérations particulières
9
9 Mode opératoire
9
9.1 Préparation de l'échantillon
9
9.2 Amplification
9
9.3 Réaction des témoins
9
9.4 Confirmation des résultats de la PCR
10
10 Évaluation
10
11 Rapport d'essai
11
Bibliographie
12

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