NF EN ISO 22959
Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons by on-line donor acceptor complex chromatography and HPLC with fluorescence detection
ISO 22959:2009 specifies a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils. The method has been validated for coconut, olive, sunflower, and soybean oil, and is possibly applicable to other oils, dependent on the determination of appropriate parameters. The lowest level of quantification for the PAHs is 0,1 µg/kg. The lowest possible amount of each PAH which can be distinguished from the baseline noise has not been determined. The validated concentration range of the method is 0,1 µg/kg to 3,5 µg/kg for each individual PAH. For samples containing (light) PAH contents > 3,5 µg/kg, dilution to bring the contents into the validated range is possible. It is also possible to adjust the range of the calibration curves. However, ranges exceeding 3,5 µg/kg have not been validated. PAHs which can be determined by this method are: anthracene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[a]anthracene, benzo[e]pyrene, benzo[a]pyrene, perylene, benzo[ghi]perylene, anthanthrene, dibenzo[a,h]anthracene, coronene, indeno[1,2,3-cd]pyrene, benzo[a]fluoranthene, benzo[b]fluoranthene, benzo[k]fluoranthene.
ISO 22959:2009 specifies a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils.
The method has been validated for coconut, olive, sunflower, and soybean oil, and is possibly applicable to other oils, dependent on the determination of appropriate parameters.
The lowest level of quantification for the PAHs is 0,1 µg/kg. The lowest possible amount of each PAH which can be distinguished from the baseline noise has not been determined. The validated concentration range of the method is 0,1 µg/kg to 3,5 µg/kg for each individual PAH. For samples containing (light) PAH contents > 3,5 µg/kg, dilution to bring the contents into the validated range is possible. It is also possible to adjust the range of the calibration curves. However, ranges exceeding 3,5 µg/kg have not been validated.
PAHs which can be determined by this method are: anthracene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[a]anthracene, benzo[e]pyrene, benzo[a]pyrene, perylene, benzo[ghi]perylene, anthanthrene, dibenzo[a,h]anthracene, coronene, indeno[1,2,3-cd]pyrene, benzo[a]fluoranthene, benzo[b]fluoranthene, benzo[k]fluoranthene.
- Avant-proposv
- Introductionvi
-
1 Domaine d'application1
-
2 Références normatives1
-
3 Termes et définitions1
-
4 Principe2
-
5 Réactifs, matériaux et étalons3
-
6 Appareillage5
-
7 Échantillonnage et préparation de l'échantillon pour essai6
-
8 Préparation des échantillons6
-
9 Mode opératoire7
-
9.1 Analyse CLHP7
-
9.2 Identification des HAP7
-
10 Calculs des différents HAP8
-
11 Données de validation de la méthode8
-
11.1 Exactitude8
-
11.2 Fidélité intralaboratoire8
-
11.3 Récupération9
-
11.4 Plage dynamique9
-
11.5 Limite de quantification9
-
12 Fidélité9
-
12.1 Essai interlaboratoires international9
-
12.2 Répétabilité9
-
12.3 Reproductibilité9
-
13 Rapport d'essai9
- Annexe A (informative) Exemple des parties constitutives d'un système de CLHP10
- Annexe B (informative) Exemple de conditions de fonctionnement des pompes du système de CLHP11
- Annexe C (informative) Exemple de conditions de fonctionnement du thermostat de colonne et du détecteur du système de CLHP12
- Annexe D (informative) Exemple de programme de l'échantillonneur automatique du système de CLHP13
- Annexe E (informative) Raccords de tubulures du système de CLHP14
- Annexe F (informative) Chromatogramme d'un échantillon étalon15
- Annexe G (informative) Fidélité de la détermination pour une huile de tournesol, plage allant de 0,1 microgramme/kg à 3,5 microgrammes/kg16
- Annexe H (informative) Fidélité de la détermination pour une huile d'olive, plage allant de 0,1 microgramme/kg à 3,5 microgrammes/kg17
- Annexe I (informative) Fidélité de la détermination pour une huile de soja, plage allant de 0,1 microgramme/kg à 3,5 microgrammes/kg18
- Annexe J (informative) Fidélité de la détermination pour une huile de coco, plage allant de 0,1 microgramme/kg à 3,5 microgrammes/kg19
- Annexe K (informative) Essai interlaboratoires20
- Bibliographie22
The Requirements department helps you quickly locate within the normative text:
- mandatory clauses to satisfy,
- non-essential but useful clauses to know, such as permissions and recommendations.
The identification of these types of clauses is based on the document “ISO / IEC Directives, Part 2 - Principles and rules of structure and drafting of ISO documents ”as well as on a constantly enriched list of verbal forms.
With Requirements, quickly access the main part of the normative text!
COBAZ is the simple and effective solution to meet the normative needs related to your activity, in France and abroad.
Available by subscription, CObaz is THE modular solution to compose according to your needs today and tomorrow. Quickly discover CObaz!
Request your free, no-obligation live demo
I discover COBAZ