NF EN ISO 24276

NF EN ISO 24276

November 2006
Standard Current

Foodstuffs - Methods of analysis for the detedtion of genetically modified organisms and derived products - General requirements and definitions

ISO 24276:2006 specifies how to use the standards for sampling strategies (EN/TS 21568), nucleic acid extraction (ISO 21571), qualitative nucleic acid analysis (ISO 21569) and quantitative nucleic acid analysis (ISO 21570), and their relationship in the analysis of genetically modified organisms in foodstuffs, and contains general definitions, requirements and guidelines for laboratory set-up, method validation requirements, description of methods and test reports. It has been established for food matrices, but could also be applied to other matrices (e.g. seeds, feed and plant samples from the environment).

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Main informations

Collections

National standards and national normative documents

Publication date

November 2006

Number of pages

25 p.

Reference

NF EN ISO 24276

ICS Codes

07.080   Biology. Botany. Zoology
67.050   General methods of tests and analysis for food products

Classification index

V03-024

Print number

1 - 27/10/2006

International kinship

ISO 24276:2006

European kinship

EN ISO 24276:2006
Sumary
Foodstuffs - Methods of analysis for the detedtion of genetically modified organisms and derived products - General requirements and definitions

ISO 24276:2006 specifies how to use the standards for sampling strategies (EN/TS 21568), nucleic acid extraction (ISO 21571), qualitative nucleic acid analysis (ISO 21569) and quantitative nucleic acid analysis (ISO 21570), and their relationship in the analysis of genetically modified organisms in foodstuffs, and contains general definitions, requirements and guidelines for laboratory set-up, method validation requirements, description of methods and test reports.

It has been established for food matrices, but could also be applied to other matrices (e.g. seeds, feed and plant samples from the environment).

Replaced standards (1)
XP V03-020-1
September 2003
Standard Cancelled
Foodstuffs - Detection and quantification of genetically modified vegetal organisms and derived products - Part 1 : guidelines and requirements for methods based on the detection of nucleic acids

Le présent document a pour objectif d'homogénéiser les résultats obtenus dans les laboratoires par la définition d'un cadre général auquel doit satisfaire toute méthode de détection des organismes végétaux génétiquement modifiés (OGM) basée sur les acides nucléiques et plus particulièrement sur la technique PCR (réaction de polymérisation en chaîne).

Table of contents
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  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    1
  • 3.1 Définitions générales
    1
  • 3.2 Termes relatifs à l'extraction et à la purification de l'ADN
    5
  • 3.3 Termes relatifs à l'amplification de l'ADN et à la PCR
    5
  • 3.4 Définitions relatives aux témoins d'ADN et de PCR
    6
  • 3.5 Termes relatifs aux produits de référence
    7
  • 3.6 Termes relatifs à la quantification
    7
  • 3.7 Termes relatifs aux OGM
    7
  • 4 Application aux Normes internationales appropriées
    7
  • 4.1 Généralités
    7
  • 4.2 Guide pour l'utilisateur quant au choix des méthodes
    8
  • 4.3 Caractéristiques de performance
    9
  • 5 Exigences générales de laboratoire et exigences relatives aux modes opératoires
    10
  • 5.1 Généralités
    10
  • 5.2 Utilisation des témoins
    11
  • 5.3 Organisation du laboratoire
    13
  • 6 Interprétation et expression des résultats
    14
  • 6.1 Généralités
    14
  • 6.2 Interprétation des témoins
    14
  • 6.3 Expression d'un résultat négatif
    15
  • 6.4 Expression d'un résultat positif
    15
  • 6.5 Expression de résultats ambigus
    15
  • 6.6 Exigences relatives à l'assurance qualité
    16
  • 7 Rapport d'essai
    16
  • Bibliographie
    17
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