NF EN ISO 4833-1

NF EN ISO 4833-1

October 2013
Standard Current

Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1 : colony-count at 30 °C by the pour plate technique

ISO 4833-1:2013 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after aerobic incubation at 30 °C. The method is applicable to: a) products intended for human consumption and for animal feed; b) environmental samples in the area of food and feed production and handling. ISO 4833-1:2013 is applicable to: 1) products that require a reliable count when a low limit of detection is specified (below 102/g or 102/ml for liquid samples or below 103/g for solid samples); 2) products expected to contain spreading colonies that obscure colonies of other organisms, e.g. milk and milk products likely to contain spreading Bacillus spp. The applicability of ISO 4833-1:2013 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in those products are not detected effectively. For some matrices, the method specified in ISO 4833-1:2013 can give different results to those obtained using the method specified in ISO 4833‑2.

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Main informations

Collections

National standards and national normative documents

Publication date

October 2013

Number of pages

21 p.

Reference

NF EN ISO 4833-1

ICS Codes

07.100.30   Food microbiology

Classification index

V08-011-1

Print number

1 - 23/10/2013

International kinship

ISO 4833-1:2013

European kinship

EN ISO 4833-1:2013
Sumary
Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1 : colony-count at 30 °C by the pour plate technique

ISO 4833-1:2013 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after aerobic incubation at 30 °C. The method is applicable to: a) products intended for human consumption and for animal feed; b) environmental samples in the area of food and feed production and handling.

ISO 4833-1:2013 is applicable to: 1) products that require a reliable count when a low limit of detection is specified (below 102/g or 102/ml for liquid samples or below 103/g for solid samples); 2) products expected to contain spreading colonies that obscure colonies of other organisms, e.g. milk and milk products likely to contain spreading Bacillus spp.

The applicability of ISO 4833-1:2013 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in those products are not detected effectively.

For some matrices, the method specified in ISO 4833-1:2013 can give different results to those obtained using the method specified in ISO 4833‑2.

Replaced standards (1)
NF EN ISO 4833
May 2003
Standard Cancelled
Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of microorganisms - Colony-count technique at 30° C

<p>ISO 4833:2003 specifies a horizontal method for the enumeration of microorganisms, by counting the colonies growing in a solid medium after aerobic incubation at 30 degrees Celsius. Subject to some limitations, it is applicable to products intended for human consumption or the feeding of animals.</p> <p>The applicability of ISO 4833:2003 to the examination of certain fermented food and animal feeding stuffs is limited. For the examination of fermented food and animal feeding stuffs, other media and/or incubation conditions might be more appropriate.</p>

Table of contents
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  • Avant-propos
    iv
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    2
  • 4 Principe
    2
  • 5 Milieux de culture et diluants
    2
  • 5.1 Généralités
    2
  • 5.2 Diluants
    2
  • 5.3 Milieu gélosé: gélose pour dénombrement (PCA)
    2
  • 5.4 Milieu pour seconde couche
    3
  • 6 Appareillage
    4
  • 7 Échantillonnage
    4
  • 8 Préparation de l'échantillon pour essai
    4
  • 9 Mode opératoire
    5
  • 9.1 Prise d'essai, suspension mère et dilutions
    5
  • 9.2 Ensemencement et incubation
    5
  • 9.3 Dénombrement des colonies
    5
  • 10 Expression des résultats
    6
  • 10.1 Méthode de calcul
    6
  • 10.2 Fidélité
    6
  • 10.3 Interprétation des résultats d'essai
    6
  • 11 Rapport d'essai
    7
  • Annexe A (informative) Utilisation de la différence critique pour l'interprétation des résultats
    8
  • Bibliographie
    9
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