NF EN ISO 6888-2

NF EN ISO 6888-2

October 1999
Standard Cancelled

Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species). Part 2 : technique using rabbit plasma fibrinogen agar

La présente partie de l'ISO 6888 spécifie une méthode horizontale pour le dénombrement des staphylocoques à coagulase positive dans les produits destinés à la consommation humaine ou à l'alimentation animale, par comptage des colonies obtenues en milieu solide (milieu au plasma de lapin et au fibrinogène) après incubation en aérobiose à 35°C ou 37°C.

View the extract
Main informations

Collections

National standards and national normative documents

Publication date

October 1999

Number of pages

15 p.

Reference

NF EN ISO 6888-2

ICS Codes

07.100.30   Food microbiology

Classification index

V08-014-2

Print number

1

International kinship

European kinship

EN ISO 6888-2:1999
Sumary
Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species). Part 2 : technique using rabbit plasma fibrinogen agar

La présente partie de l'ISO 6888 spécifie une méthode horizontale pour le dénombrement des staphylocoques à coagulase positive dans les produits destinés à la consommation humaine ou à l'alimentation animale, par comptage des colonies obtenues en milieu solide (milieu au plasma de lapin et au fibrinogène) après incubation en aérobiose à 35°C ou 37°C.

Replaced standards (1)
NF V08-014
January 1984
Standard Cancelled
Microbiology of food products. General guidance for enumeration of {Staphylococcus} aureus. Colony count technique.

Standard replaced by (1)
NF EN ISO 6888-2
September 2021
Standard Current
Microbiology of the food chain - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 2 : method using rabbit plasma fibrinogen agar medium

<p>This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (rabbit plasma fibrinogen agar medium) after aerobic incubation at 34 °C to 38 °C (see Reference [10]).</p> <p>This document is applicable to:</p> <p>—    products intended for human consumption;</p> <p>—    products intended for animal feeding;</p> <p>—    environmental samples in the area of food and feed production and handling;</p> <p>—    samples from the primary production stage.</p> <p>This horizontal method was originally developed for the examination of all samples belonging to the food chain.</p> <p>Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.</p> <p>Based on the information available at the time of publication of this document, this method is not considered to be (fully) suited to the examination of fermented products or other products containing technological flora based on <em>Staphylococcus</em> spp. (e.g. <em>S. xylosus</em>) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by:</p> <p>—    staphylococci forming atypical colonies on a Baird-Parker agar medium;</p> <p>—    background flora that can obscure the colonies being sought.</p> <p>Nevertheless, both ISO 6888-1 and this document are given equivalent status.</p>

Table of contents
View the extract
  • 1
    Domaine d'application 1
  • 2
    Références normatives 1
  • 3
    Termes et définitions 1
  • 4
    Principe 2
  • 5
    Diluant et milieux de culture 2
  • 6
    Appareillage et verrerie 3
  • 7
    Échantillonnage 4
  • 8
    Préparation de l'échantillon pour essai 4
  • 9
    Mode opératoire 4
  • 10
    Expression des résultats 5
  • 11
    Fidélité 6
  • 12
    Rapport d'essai 6
  • Bibliographie
    7
Need to identify, monitor and decipher standards?

COBAZ is the simple and effective solution to meet the normative needs related to your activity, in France and abroad.

Available by subscription, CObaz is THE modular solution to compose according to your needs today and tomorrow. Quickly discover CObaz!

Request your free, no-obligation live demo

I discover COBAZ