NF ISO 16649-1

NF ISO 16649-1

September 2018
Standard Current

Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 1 : colony-count technique at 44 °C using membranes and 5-bromo-4-chloro-3-indolyl beta-D-glucuronide

ISO 16649-1:2018 specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli by colony-count technique after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme β-glucuronidase[9][10][13][14][17][18][19][20]. It is applicable to - products intended for human consumption, - products intended for feeding animals, - environmental samples in the area of food production and food handling, and - samples from the primary production stage such as animal faeces, dust, and swabs.

See more
View the extract
Main informations

Collections

National standards and national normative documents

Publication date

September 2018

Number of pages

17 p.

Reference

NF ISO 16649-1

ICS Codes

07.100.30   Food microbiology

Classification index

V08-031-1

Print number

1

International kinship

ISO 16649-1:2018
Sumary
Microbiology of the food chain - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 1 : colony-count technique at 44 °C using membranes and 5-bromo-4-chloro-3-indolyl beta-D-glucuronide

ISO 16649-1:2018 specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli by colony-count technique after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme β-glucuronidase[9][10][13][14][17][18][19][20]. It is applicable to

- products intended for human consumption,

- products intended for feeding animals,

- environmental samples in the area of food production and food handling, and

- samples from the primary production stage such as animal faeces, dust, and swabs.

Replaced standards (1)
NF ISO 16649-1
August 2001
Standard Cancelled
Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of bêta-glucuronidase positive Escherichia coli - Part 1 : colony-count technique at 44° C using membranes and 5-bromo-4-chloro-3-indolyl beta-D-glucoronide

<p> La présente partie de l'ISO 16649 spécifie une méthode horizontale pour le dénombrement des Escherichia coli bêta-glucuronidase positive dans des produits alimentaires destinés à la consommation humaine ou à l'alimentation des animaux. Elle utilise une technique de comptage des colonies après revivification au moyen de membranes puis incubation à 44 °C sur un milieu solide contenant un substrat chromogénique pour la détection de l'enzyme bêta-glucuronidase. AVERTISSEMENT - Certaines souches d'Escherichia coli qui ne poussent pas à 44 °C et, en particulier, celles qui sont bêta-glucuronidase négative, telles que les Escherichia coli O157, ne peuvent pas être mises en évidence. </p>

Table of contents
View the extract
  • Avant-propos
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Milieux de culture et réactifs
  • 6 Matériel et consommables
  • 7 Prélèvement
  • 8 Préparation de l'échantillon pour essai
  • 9 Mode opératoire
  • 10 Expression des résultats
  • 11 Caractéristiques de performance de la méthode
  • 12 Rapport d'essai
  • 13 Assurance qualité
  • Annexe A (normative) Composition et préparation des milieux de culture et des réactifs
  • Bibliographie
ZOOM ON ... the Requirements department
To comply with a standard, you need to quickly understand its issues in order to determine its impact on your activity.

The Requirements department helps you quickly locate within the normative text:
- mandatory clauses to satisfy,
- non-essential but useful clauses to know, such as permissions and recommendations.

The identification of these types of clauses is based on the document “ISO / IEC Directives, Part 2 - Principles and rules of structure and drafting of ISO documents ”as well as on a constantly enriched list of verbal forms.

With Requirements, quickly access the main part of the normative text!

With Requirements, quickly access the main part of the normative text!
What is the Redline format?
The Redline + service - standards comparator allows you to easily and simply identify major changes between the current standard and its last canceled version.

At a glance, you will be able to identify the additions, deletions or modifications to a text, table, figure and formula.
At a glance, you will be able to identify the additions, deletions or modifications to a text, table, figure and formula

The Redlines + service is offered to you on the collection of French standards in force, in French language and in HTML and PDF format.

For an overview of the service, click on View a standard in redline format
Need to identify, monitor and decipher standards?

COBAZ is the simple and effective solution to meet the normative needs related to your activity, in France and abroad.

Available by subscription, CObaz is THE modular solution to compose according to your needs today and tomorrow. Quickly discover CObaz!

Request your free, no-obligation live demo

I discover COBAZ