NF ISO 17244
Water quality - Determination of the toxicity of water samples on the embryo-larval development of Japanese oyster (Crassostrea gigas) and mussel (Mytilus edulis or Mytilus galloprovincialis)
ISO 17244:2015 specifies a method for determining the effects of chemical and aqueous samples on the embryo-larval development of marine bivalves. It allows the determination of the concentration levels that result in an abnormality in embryo-larval development. This test is suitable for salinity ranges between 20 and 40 for mussels and between 25 and 35 for oysters. This method applies to - chemical substances and preparations, - marine and brackish waters, - streams and aqueous effluents (urban, agricultural, industrial effluents, etc.) as long as the salinity is adjusted and/or dilution is limited so that the aforementioned salinity ranges are respected, and - aqueous extracts (pore water, elutriates, eluates, and leachates) from sediments and petroleum products.
ISO 17244:2015 specifies a method for determining the effects of chemical and aqueous samples on the embryo-larval development of marine bivalves. It allows the determination of the concentration levels that result in an abnormality in embryo-larval development. This test is suitable for salinity ranges between 20 and 40 for mussels and between 25 and 35 for oysters. This method applies to
- chemical substances and preparations,
- marine and brackish waters,
- streams and aqueous effluents (urban, agricultural, industrial effluents, etc.) as long as the salinity is adjusted and/or dilution is limited so that the aforementioned salinity ranges are respected, and
- aqueous extracts (pore water, elutriates, eluates, and leachates) from sediments and petroleum products.
Le présent document présente un essai basé sur l'emploi de la phase du développement embryo-larvaire de bivalve (huître creuse ou moule) pour évaluer la toxicité potentielle d'une substance ou d'un échantillon aqueux présent dans le milieu marin. Il est aussi possible de rechercher la toxicité potentielle d'échantillons d'eaux estuariennes, de cours d'eau, d'effluents aqueux (urbains, agricoles, industriels ) ou d'extrait aqueux de sédiments ou de produits pétroliers. Il porte sur les phases de développement larvaire allant de l'oeuf fécondé au stade larve D : exposition à de substances et composés toxiques pouvant induire des effets tératogènes sublétaux. Les gammes de pH, salinité et température acceptées par les bivalves permettent leur emploi aisé dans les études d'écotoxicité en particulier pour l'évaluation de la qualité des milieux côtiers et estuariens.
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