XP CEN ISO/TS 20836

XP CEN ISO/TS 20836

May 2007
Standard Cancelled

Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Performance testing for thermal cyclers

ISO/TS 20836:2005 provides basic requirements for the installation, performance and maintenance of thermal cyclers. Although thermal cyclers are robust technical equipment, they do require regular maintenance. Their cooling/heating elements have a limited lifetime. Proper functioning of the cooling/heating element depends both on the quality of the cooling/heating devices and proper use and care. In addition to outlining the requirement for a defined maintenance programme, procedures are described for the determination of thermal cycler performance by biochemical or physical methods.

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Main informations

Collections

National standards and national normative documents

Publication date

May 2007

Number of pages

24 p.

Reference

XP CEN ISO/TS 20836

ICS Codes

07.100.30   Food microbiology

Classification index

V03-027

Print number

1 - 07/06/2007

International kinship

European kinship

CEN ISO/TS 20836:2005
Sumary
Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Performance testing for thermal cyclers

ISO/TS 20836:2005 provides basic requirements for the installation, performance and maintenance of thermal cyclers. Although thermal cyclers are robust technical equipment, they do require regular maintenance. Their cooling/heating elements have a limited lifetime. Proper functioning of the cooling/heating element depends both on the quality of the cooling/heating devices and proper use and care.

In addition to outlining the requirement for a defined maintenance programme, procedures are described for the determination of thermal cycler performance by biochemical or physical methods.

Standard replaced by (1)
NF EN ISO 20836
December 2021
Standard Current
Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of microorganisms - Thermal performance testing of thermal cyclers

<p>This document specifies requirements for the installation, maintenance, temperature calibration and temperature performance testing of standard thermal cyclers and real-time thermal cyclers. It is applicable to the detection of microorganisms as well as any other applications in the food chain using polymerase chain reaction (PCR)-based methods.</p> <p>This document has been established for food testing, but is also applicable to other domains using thermal cyclers (e.g. environmental, human health, animal health, forensic testing).</p>

Table of contents
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  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    1
  • 4 Installation des thermocycleurs
    2
  • 5 Maintenance des thermocycleurs
    2
  • 6 Essais de performance
    2
  • 6.1 Généralités
    2
  • 6.2 Essai biochimique de performance
    3
  • 6.3 Essai physique de performance
    3
  • 7 Rapport d'essai et relevé des écarts
    3
  • Annexe A (informative) Essai biochimique de performance - Méthode de PCR pour le contrôle de l'exactitude des températures
    4
  • Annexe B (informative) Essai physique de performance
    12
  • Bibliographie
    14
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