XP CEN ISO/TS 6579-2
Microbiology of food and animal feed - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 2 : enumeration by a miniaturized most probable number technique
ISO/TS 6579-2 gives a method for the enumeration of Salmonella spp. present in: products intended for human consumption and for the feeding of animals; environmental samples in the area of food production and food handling; animal faeces; and environmental samples from the primary production stage by calculation of the most probable number (MPN). The method is based on miniaturization of the dilution, pre-enrichment and selective enrichment steps. The selective enrichment medium, modified semi-solid Rappaport?Vassiliadis (MSRV), is intended for the detection of motile salmonellae and is not appropriate for the detection of non-motile salmonellae. It is possible that the method is less appropriate to enumerate Salmonella ser. Typhi and Salmonella ser. Paratyphi. The method is not appropriate for the enumeration of Salmonella spp. in (very) low contaminated samples (<1 cfu/g).
ISO/TS 6579-2 gives a method for the enumeration of Salmonella spp. present in: products intended for human consumption and for the feeding of animals; environmental samples in the area of food production and food handling; animal faeces; and environmental samples from the primary production stage by calculation of the most probable number (MPN).
The method is based on miniaturization of the dilution, pre-enrichment and selective enrichment steps. The selective enrichment medium, modified semi-solid Rappaport?Vassiliadis (MSRV), is intended for the detection of motile salmonellae and is not appropriate for the detection of non-motile salmonellae.
It is possible that the method is less appropriate to enumerate Salmonella ser. Typhi and Salmonella ser. Paratyphi.
The method is not appropriate for the enumeration of Salmonella spp. in (very) low contaminated samples (<1 cfu/g).
- Avant-proposiv
- Introductionv
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1 Domaine d'application1
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2 Références normatives2
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3 Termes et définitions2
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4 Principe2
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4.1 Généralités2
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4.2 Préenrichissement en milieu liquide non sélectif2
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4.3 Enrichissement en milieu semi-solide sélectif2
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4.4 Isolement sélectif et identification3
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4.5 Confirmation3
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4.6 Calcul du NPP3
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5 Milieux de culture et sérums3
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5.1 Généralités3
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5.2 Milieux de culture3
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6 Appareillage4
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7 Échantillonnage4
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8 Préparation de l'échantillon pour essai4
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9 Mode opératoire5
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9.1 Prise d'essai et suspension mère5
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9.2 Dilution et préenrichissement en milieu liquide non sélectif5
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9.3 Enrichissement sélectif en milieu semi-solide5
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9.4 Isolement sélectif6
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9.5 Confirmation biochimique et sérologique6
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10 Expression des résultats8
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11 Rapport d'essai8
- Annexe A (informative) Composition et préparation de milieux de culture et de réactifs10
- Annexe B (informative) Schéma du mode opératoire17
- Bibliographie18
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