NF EN ISO 11733
Water quality - Determination of the elimination and biodegradability of organic compounds in an aqueous medium - Activated sludge simulation test
ISO 11733:2004 specifies a method for the determination of the elimination and the biodegradability of organic compounds by aerobic micro-organisms. The conditions described simulate a wastewater treatment plant. Two test systems can be used: activated sludge plants or porous pots. The tests can optionally be performed under conditions of nitrification and denitrification and coupling of the units. The method applies to organic compounds which, under the conditions of the test, are soluble in tap water at the test concentration and not expected to be transformed to insoluble metabolites if biodegradation, in addition to elimination, is determined;poorly water-soluble, but which are satisfactorily dispersible in water and allow detection with suitable analytical means (e.g. organic carbon measurements);non-volatile, or which have a negligible vapour pressure under the test conditions;not inhibitory to the test micro-organisms at the concentration chosen for the test. Compounds inhibitory at concentrations used in this test may be tested at concentrations less than their EC20 value, followed by higher practical concentrations after a period of acclimatization. The method can also be used to measure the biodegradation and elimination of dissolved organic compounds in waste water (also called "test compound" in the method).
ISO 11733:2004 specifies a method for the determination of the elimination and the biodegradability of organic compounds by aerobic micro-organisms. The conditions described simulate a wastewater treatment plant. Two test systems can be used: activated sludge plants or porous pots. The tests can optionally be performed under conditions of nitrification and denitrification and coupling of the units.
The method applies to organic compounds which, under the conditions of the test, are
- soluble in tap water at the test concentration and not expected to be transformed to insoluble metabolites if biodegradation, in addition to elimination, is determined;
- poorly water-soluble, but which are satisfactorily dispersible in water and allow detection with suitable analytical means (e.g. organic carbon measurements);
- non-volatile, or which have a negligible vapour pressure under the test conditions;
- not inhibitory to the test micro-organisms at the concentration chosen for the test. Compounds inhibitory at concentrations used in this test may be tested at concentrations less than their EC20 value, followed by higher practical concentrations after a period of acclimatization.
The method can also be used to measure the biodegradation and elimination of dissolved organic compounds in waste water (also called "test compound" in the method).
- Avant-proposiv
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1 Domaine d'application1
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2 Références normatives1
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3 Termes et définitions2
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4 Principe3
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5 Environnement d'essai4
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6 Réactifs4
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7 Appareillage7
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8 Mode opératoire8
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8.1 Généralités8
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8.2 Préparation de l'inoculum9
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8.3 Réalisation de l'essai9
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9 Calcul et expression des résultats12
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9.1 Calcul du taux d'élimination12
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9.2 Expression des résultats13
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9.3 Indication de la biodégradation13
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9.4 Biodégradation du milieu organique13
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10 Validité de l'essai13
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11 Rapport d'essai14
- Annexe A (informative) Modification de l'essai de simulation des boues activées pour les unités de traitement des eaux résiduaires par nitrification-dénitrification15
- Annexe B (informative) Couplage des unités d'essai (facultatif)19
- Annexe C (informative) Systèmes d'essai20
- Annexe D (informative) Effets du temps de rétention des boues sur la concentration de l'effluent23
- Annexe E (informative) Exemple d'une courbe d'élimination/dégradation27
- Bibliographie28
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