NF EN ISO 12228-1

NF EN ISO 12228-1

August 2014
Standard Current

Determination of individual and total sterols contents - Gas chromatographic method - Part 1 : animal and vegetable fats and oils

ISO 12228-1:2014 specifies a procedure for the gas chromatographic determination of the content and composition of sterols in animal and vegetable fats and oils. However, the determination of the contents and composition of sterols in olive and olive pomace oils is to be carried out using ISO 12228-2.

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Main informations

Collections

National standards and national normative documents

Publication date

August 2014

Number of pages

29 p.

Reference

NF EN ISO 12228-1

ICS Codes

67.200.10   Animal and vegetable fats and oils

Classification index

T60-258-1

Print number

1

International kinship

ISO 12228-1:2014

European kinship

EN ISO 12228-1:2014
Sumary
Determination of individual and total sterols contents - Gas chromatographic method - Part 1 : animal and vegetable fats and oils

ISO 12228-1:2014 specifies a procedure for the gas chromatographic determination of the content and composition of sterols in animal and vegetable fats and oils. However, the determination of the contents and composition of sterols in olive and olive pomace oils is to be carried out using ISO 12228-2.

Replaced standards (1)
NF EN ISO 12228
May 1999
Standard Cancelled
Animal and vegetable fats and oils. Determination of individual and total sterols contents. Gas chromatographic method.

Le présent document prescrit une méthode de détermination de la teneur et de la composition des stérols contenus dans les corps gras par chromatographie en phase gazeuse. Les stérols sont l'un des principaux constituants de la fraction insaponifiable des corps gras d'origines animale et végétale et présentent une composition relative assez spécifique pour chacun d'entre eux.

Table of contents
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  • Avant-propos
    iv
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    1
  • 4 Principe
    1
  • 5 Réactifs
    2
  • 6 Appareillage
    3
  • 7 Échantillon
    3
  • 7.1 Échantillonnage
    3
  • 7.2 Préparation de l'échantillon pour essai
    4
  • 8 Mode opératoire
    4
  • 8.1 Préparation de la colonne d'oxyde d'aluminium
    4
  • 8.2 Prise d'essai
    4
  • 8.3 Extraction de l'insaponifiable
    4
  • 8.4 Chromatographie sur couche mince
    4
  • 8.5 Isolement des stérols
    4
  • 8.6 Préparation des triméthylsilyléthers stéroliques
    5
  • 8.7 Chromatographie en phase gazeuse
    5
  • 9 Expression des résultats
    5
  • 9.1 Identification des stérols
    5
  • 9.2 Composition des stérols
    6
  • 9.3 Détermination de la teneur en stérols totaux
    6
  • 10 Fidélité
    7
  • 10.1 Essai interlaboratoires
    7
  • 10.2 Limite de répétabilité, r
    7
  • 10.3 Limite de reproductibilité, R
    7
  • 11 Rapport d'essai
    7
  • Annexe A (informative) Figures
    9
  • Annexe B (informative) Essai interlaboratoires
    16
  • Bibliographie
    24
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