NF EN ISO 15216-2

NF EN ISO 15216-2

September 2019
Standard Current

Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 2 : method for detection

This document specifies a method for detection of hepatitis A virus (HAV) and norovirus genogroups I (GI) and II (GII), from test samples of foodstuffs [(soft fruit, leaf, stem and bulb vegetables, bottled water, bivalve molluscan shellfish (BMS)] or surfaces using real-time RT-PCR. This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, surfaces or other matrices.

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Main informations

Collections

National standards and national normative documents

Publication date

September 2019

Number of pages

50 p.

Reference

NF EN ISO 15216-2

ICS Codes

07.100.30   Food microbiology

Classification index

V08-670-2

Print number

1

International kinship

ISO 15216-2:2019

European kinship

EN ISO 15216-2:2019
Sumary
Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 2 : method for detection

This document specifies a method for detection of hepatitis A virus (HAV) and norovirus genogroups I (GI) and II (GII), from test samples of foodstuffs [(soft fruit, leaf, stem and bulb vegetables, bottled water, bivalve molluscan shellfish (BMS)] or surfaces using real-time RT-PCR.

This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, surfaces or other matrices.

Replaced standards (1)
XP CEN ISO/TS 15216-2
June 2013
Standard Cancelled
Microbiology of food and animal feed - Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR - Part 2 : Method for qualitative detection

<p>ISO/TS 15216-2:2013 describes a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.</p> <p>This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.</p>

Table of contents
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  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Réactifs
  • 6 Équipement et consommables
  • 7 Échantillonnage
  • 8 Mode opératoire
  • 9 Interprétation des résultats
  • 10 Expression des résultats
  • 11 Caractéristiques de performance de la méthode
  • 12 Rapport d'essai
  • Annexe A Diagramme de mode opératoire
  • Annexe B Composition et préparation des réactifs et des tampons
  • Annexe C Mélanges réactionnels de RT-PCR en temps réel et paramètres de cycles
  • Annexe D Amorces et sondes d'hydrolyse de la RT-PCR en temps réel pour la détection du VHA, du norovirus GI et GII et du mengovirus (témoin de processus)
  • Annexe E Multiplication de la souche du mengovirus MC0 utilisé comme témoin de processus
  • Annexe F Extraction d'ARN avec le système NucliSens 3 3 BioMerieux NucliSens est l'appellation commerciale d'un produit fourni par BioMerieux. Cette information est donnée à l'intention des utilisateurs du présent document et ne signifie nullement que l'ISO approuve ou recommande l'emploi exclusif du produit ainsi désigné. Des produits équivalents peuvent être utilisés s'il est démontré qu'ils conduisent aux mêmes résultats. de chez BioMérieux
  • Annexe G Solutions mères d'ARN contrôle externe (ARN CE)
  • Annexe H Disposition de plaque optique type
  • Annexe I Études de validation de la méthode et caractéristiques de performances
  • Bibliographie
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