NF EN ISO 21479

NF EN ISO 21479

May 2020
Standard Current

Soil quality - Determination of the effects of pollutants on soil flora - Leaf fatty acid composition of plants to assess soil quality

This document describes a method to compare the quality of soils by determining the fatty acid composition of the leaves of plant species grown in these soils. This method does not make it possible to determine an optimal value of the Omega-3 index and, therefore, cannot be used to determine the intrinsic quality of a soil from a specific area (regarded as homogeneous). The method can only be used to compare the quality of soils between various areas. This method is applicable to: — soils from contaminated sites; — amended soils; — soils after remediation; ? soil with waste products (e.g. slurry, manure, sludge or composts). Alternatively, the quality of soils can be assessed by determining the Omega-3 index of Lactuca sativa seedlings grown in these soils under controlled conditions (i.e. phytotronic chamber) and by comparing these values to those obtained from control soils (see Annex B).

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Main informations

Collections

National standards and national normative documents

Publication date

May 2020

Number of pages

32 p.

Reference

NF EN ISO 21479

ICS Codes

13.080.30   Biological properties of soils

Classification index

X31-328

Print number

1

International kinship

ISO 21479:2019

European kinship

EN ISO 21479:2020
Sumary
Soil quality - Determination of the effects of pollutants on soil flora - Leaf fatty acid composition of plants to assess soil quality

This document describes a method to compare the quality of soils by determining the fatty acid composition of the leaves of plant species grown in these soils.

This method does not make it possible to determine an optimal value of the Omega-3 index and, therefore, cannot be used to determine the intrinsic quality of a soil from a specific area (regarded as homogeneous). The method can only be used to compare the quality of soils between various areas.

This method is applicable to:

— soils from contaminated sites;

— amended soils;

— soils after remediation;

? soil with waste products (e.g. slurry, manure, sludge or composts).

Alternatively, the quality of soils can be assessed by determining the Omega-3 index of Lactuca sativa seedlings grown in these soils under controlled conditions (i.e. phytotronic chamber) and by comparing these values to those obtained from control soils (see Annex B).

Replaced standards (1)
NF ISO 21479
September 2019
Standard Cancelled
Soil quality - Determination of the effects of pollutants on soil flora - Leaf fatty acid composition of plants used to assess soil quality

<p>This document describes a method to compare the quality of soils by determining the fatty acid composition of the leaves of plant species grown in these soils.</p> <p>This method does not make it possible to determine an optimal value of the Omega-3 index and, therefore, cannot be used to determine the intrinsic quality of a soil from a specific area (regarded as homogeneous). The method can only be used to compare the quality of soils between various areas.</p> <p>This method is applicable to:</p> <p>— soils from contaminated sites;</p> <p>— amended soils;</p> <p>— soils after remediation;</p> <p>? soil with waste products (e.g<i>.</i> slurry, manure, sludge or composts).</p> <p>Alternatively, the quality of soils can be assessed by determining the Omega-3 index of <i>Lactuca sativa</i> seedlings grown in these soils under controlled conditions (i.e. phytotronic chamber) and by comparing these values to those obtained from control soils (see Annex B).</p>

Table of contents
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  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes, définitions et abréviations
  • 4 Principe
  • 5 Appareillage et réactifs
  • 6 Stratégies d'échantillonnage
  • 7 Échantillonnage des tissus foliaires
  • 8 Obtention, extraction et analyses des FAME
  • 9 Rapport d'essai
  • Annexe A Résultats de l'essai interlaboratoires
  • Annexe B Évaluation de la qualité du sol par détermination de l'indice Oméga-3 de plantules de Lactuca sativa cultivées ex situ dans des conditions contrôlées
  • Annexe C Espèces végétales utilisées précédemment avec succès pour évaluer les sols de sites contaminés (par des composés organiques et/ou des métaux)
  • Annexe D Variation de l'indice Oméga-3 en fonction de l'heure de récolte, de la taille des végétaux et du développement foliaire
  • Annexe E Effet de la quantité de tissus foliaires sur la composition en FAME
  • Annexe F Exemple de chromatogramme obtenu après analyse des FAME de tissus foliaires
  • Annexe G Méthode mathématique recommandée pour noter les sols de zones lorsque certaines espèces végétales échantillonnées ne sont pas trouvées dans toutes les zones
  • Bibliographie
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