NF EN ISO 23753-2

NF EN ISO 23753-2

October 2011
Standard Cancelled

Soil quality - Determination of dehydrogenase activity in soils - Part 2 : method using iodotetrazolium chloride (INT)

ISO 23753-2:2005 specifies a method for determining soil dehydrogenase activity using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT). As the INT reduction is less sensitive to O2, the method is more reproducible than the TTC-method described in ISO 23753-1. It is not applicable for determining the dehydrogenase activity in upper layers (L, F, H horizons) of forest humus forms with low microbial activity (e.g. mor), or in soils showing reducing properties (e.g. waterlogged soils).

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Main informations

Collections

National standards and national normative documents

Publication date

October 2011

Number of pages

13 p.

Reference

NF EN ISO 23753-2

ICS Codes

13.080.30   Biological properties of soils

Classification index

X31-293-2

Print number

1 - 19/09/2011

International kinship

ISO 23753-2:2005

European kinship

EN ISO 23753-2:2011
Sumary
Soil quality - Determination of dehydrogenase activity in soils - Part 2 : method using iodotetrazolium chloride (INT)

ISO 23753-2:2005 specifies a method for determining soil dehydrogenase activity using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT). As the INT reduction is less sensitive to O2, the method is more reproducible than the TTC-method described in ISO 23753-1. It is not applicable for determining the dehydrogenase activity in upper layers (L, F, H horizons) of forest humus forms with low microbial activity (e.g. mor), or in soils showing reducing properties (e.g. waterlogged soils).

Standard replaced by (1)
NF EN ISO 23753-2
March 2019
Standard Current
Soil quality - Determination of dehydrogenases activity in soils - Part 2 : method using iodotetrazolium chloride (INT)

<p>This document specifies a method for determining activity of dehydrogenases in soil, using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT)<sup>[1]-[5]</sup>. As the INT reduction is less sensitive to O2, the method is more robust than the TTC-method described in ISO 23753-1.</p>

Table of contents
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  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Principe
    1
  • 4 Réactifs et produits
    2
  • 5 Appareillage
    3
  • 6 Mode opératoire
    3
  • 7 Calculs
    3
  • 8 Rapport d'essai
    4
  • Bibliographie
    5
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