XP CEN ISO/TS 17919

XP CEN ISO/TS 17919

December 2013
Standard Current

Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Detection of botulinum type A, B, E and F neurotoxin- producing clostridia

ISO/TS 17919:2013 specifies a horizontal method for the molecular detection of clostridia carrying botulinum neurotoxin A, B, E, and F genes by a PCR method. This method detects the genes and not the toxins, therefore a positive result does not necessarily mean the presence of these toxins in the sample investigated. ISO/TS 17919:2013 is applicable to products for human consumption, animal feed, and environmental samples. The PCR assays for detection of genetic sequences encoding specific toxin types are described in annexes.

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Main informations

Collections

National standards and national normative documents

Publication date

December 2013

Number of pages

59 p.

Reference

XP CEN ISO/TS 17919

ICS Codes

07.100.30   Food microbiology

Classification index

V08-752

Print number

1 - 18/12/2013

International kinship

ISO/TS 17919:2013

European kinship

CEN ISO/TS 17919:2013
Sumary
Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Detection of botulinum type A, B, E and F neurotoxin- producing clostridia

ISO/TS 17919:2013 specifies a horizontal method for the molecular detection of clostridia carrying botulinum neurotoxin A, B, E, and F genes by a PCR method. This method detects the genes and not the toxins, therefore a positive result does not necessarily mean the presence of these toxins in the sample investigated. ISO/TS 17919:2013 is applicable to products for human consumption, animal feed, and environmental samples.

The PCR assays for detection of genetic sequences encoding specific toxin types are described in annexes.

Table of contents
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  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine dapplication
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    1
  • 4 Symboles et termes abrégés
    2
  • 4.1 Symboles
    2
  • 4.2 Termes abrégés
    2
  • 5 Principes
    2
  • 5.1 Généralités
    2
  • 5.2 Enrichissement microbien
    2
  • 5.3 Extraction des acides nucléiques
    2
  • 5.4 Amplification par PCR
    2
  • 5.5 Détection des produits PCR
    2
  • 5.6 Confirmation
    3
  • 6 Réactifs
    3
  • 6.1 Généralités
    3
  • 6.2 Milieux de culture
    3
  • 6.3 Extraction des acides nucléiques
    4
  • 6.4 Réactifs pour la PCR
    5
  • 7 Appareillage et matériel
    5
  • 7.1 Généralités
    5
  • 7.2 Matériel pour la préparation des échantillons avant enrichissement
    5
  • 7.3 Matériel pour l'enrichissement microbien
    6
  • 7.4 Matériel utilisé pour l'extraction des acides nucléiques
    6
  • 7.5 Matériel utilisé pour la PCR
    6
  • 7.6 Matériel utilisé pour la détection des produits PCR
    6
  • 8 Échantillonnage
    7
  • 9 Mode opératoire
    7
  • 9.1 Préparation de l'échantillon avant enrichissement
    7
  • 9.2 Enrichissement microbien
    7
  • 9.3 Préparation des acides nucléiques
    8
  • 9.4 Amplification par PCR
    9
  • 9.5 Confirmation d'un résultat PCR positif
    9
  • Annexe A (normative) Schéma du mode opératoire
    11
  • Annexe B (informative) Réactions de PCR multiplex pour la détection de gènes codant les types A, B, E et F de la neurotoxine botulique utilisant une électrophorèse en gel d'agarose
    12
  • Annexe C (informative) Essais pour la détection des gènes codant les types A, B, E et F de neurotoxine botulique utilisant la PCR en temps réel
    30
  • Annexe D (informative) Préparation des spores de C. botulinum
    42
  • Bibliographie
    48
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