ISO 14461-2:2005

ISO 14461-2:2005

April 2005
International standard Current

Milk and milk products - Quality control in microbiological laboratories - Part 2 : determination of the reliability of colony counts of parallel plates and subsequent dilution steps

ISO 14461-2|IDF 169-2:2005 describes a routine procedure for the evaluation of results of the enumeration of microorganisms using colony-count methods with subsequent 10-fold dilution steps and one plate or two parallel plates within each dilution step. This routine procedure is applied regularly in each laboratory performing colony counts. It provides criteria for the acceptability of differences between the results from parallel plates and subsequent dilution steps, as follows.The results (colony counts) obtained from parallel plates are compared with tabulated limits for given colony counts. If these limits are exceeded, a technical problem when performing the parallel determinations may be indicated.The results (sums of colony counts) of two parallel plates of two subsequent 10-fold dilution steps are compared with tabulated limits for given sums of colony counts. If these limits are exceeded, a technical problem when performing the dilutions may be indicated.If the limits mentioned above are exceeded in more cases than expected, this indicates that the test procedure lacks reliability.

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Main informations

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International ISO standards

Publication date

April 2005

Number of pages

18 p.

Reference

ISO 14461-2:2005

ICS Codes

07.100.30   Food microbiology

Print number

1 - 09/05/2005
Sumary
Milk and milk products - Quality control in microbiological laboratories - Part 2 : determination of the reliability of colony counts of parallel plates and subsequent dilution steps

ISO 14461-2|IDF 169-2:2005 describes a routine procedure for the evaluation of results of the enumeration of microorganisms using colony-count methods with subsequent 10-fold dilution steps and one plate or two parallel plates within each dilution step.

This routine procedure is applied regularly in each laboratory performing colony counts. It provides criteria for the acceptability of differences between the results from parallel plates and subsequent dilution steps, as follows.

The results (colony counts) obtained from parallel plates are compared with tabulated limits for given colony counts. If these limits are exceeded, a technical problem when performing the parallel determinations may be indicated.

The results (sums of colony counts) of two parallel plates of two subsequent 10-fold dilution steps are compared with tabulated limits for given sums of colony counts. If these limits are exceeded, a technical problem when performing the dilutions may be indicated.

If the limits mentioned above are exceeded in more cases than expected, this indicates that the test procedure lacks reliability.

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