NF EN ISO 11701
Vegetable fats and oils - Determination of phospholipids content in lecithins by HPLC using a light-scattering detector
ISO 11701:2009 specifies a method for the quantitative determination of phospholipids content by high performance liquid chromatography (HPLC) using a diol column and a light-scattering detector. The method is applicable to crude, oil-containing lecithins, and to oil-free lecithins and lecithin fractions from vegetable fats and oils. The method is not applicable to animal and ruminant lecithins and enzymatically hydrolysed lecithins as the peak separation of lysophosphatidylethanolamine (LPE), lysophosphatidylinositol (LPI) and lysophosphatidic acid (LPA) is insufficient.
ISO 11701:2009 specifies a method for the quantitative determination of phospholipids content by high performance liquid chromatography (HPLC) using a diol column and a light-scattering detector.
The method is applicable to crude, oil-containing lecithins, and to oil-free lecithins and lecithin fractions from vegetable fats and oils.
The method is not applicable to animal and ruminant lecithins and enzymatically hydrolysed lecithins as the peak separation of lysophosphatidylethanolamine (LPE), lysophosphatidylinositol (LPI) and lysophosphatidic acid (LPA) is insufficient.
- Avant-proposiv
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1 Domaine d'application1
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2 Références normatives1
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3 Termes et définitions1
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4 Principe1
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5 Réactifs2
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6 Appareillage2
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7 Échantillonnage3
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8 Préparation de l'échantillon pour essai3
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9 Mode opératoire3
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10 Calcul et expression des résultats5
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11 Fidélité de la méthode5
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12 Rapport d'essai6
- Annexe A (informative) Chromatogramme de CLHP7
- Annexe B (informative) Résultats d'un essai interlaboratoires8
- Bibliographie11
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