NF EN ISO 18363-4

NF EN ISO 18363-4

August 2021
Standard Current

Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 4 : method using fast alkaline transesterification and measurement for 2-MCPD, 3- MCPD and glycidol by GC-MS/MS

This document specifies a rapid procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on alkaline catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC-MS/MS analysis. Glycidyl ester overestimation is corrected by addition of an isotopic labelled ester bound 3-MCPD which allows the quantification of 3-MCPD induced glycidol during the procedure.This method is applicable to solid and liquid fats and oils. This document also applies to animal fats and used frying oils and fats, but these matrices were not included in the validation. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.Milk and milk products (or fat coming from milk and milk products), infant formulas, emulsifiers, free fatty acids and other fats- and oils-derived matrices are excluded from the scope of this document.

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Main informations

Collections

National standards and national normative documents

Publication date

August 2021

Number of pages

32 p.

Reference

NF EN ISO 18363-4

ICS Codes

67.200.10   Animal and vegetable fats and oils

Classification index

T60-285-4

Print number

1

International kinship

ISO 18363-4:2021

European kinship

EN ISO 18363-4:2021
Sumary
Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 4 : method using fast alkaline transesterification and measurement for 2-MCPD, 3- MCPD and glycidol by GC-MS/MS

This document specifies a rapid procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on alkaline catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC-MS/MS analysis. Glycidyl ester overestimation is corrected by addition of an isotopic labelled ester bound 3-MCPD which allows the quantification of 3-MCPD induced glycidol during the procedure.

This method is applicable to solid and liquid fats and oils. This document also applies to animal fats and used frying oils and fats, but these matrices were not included in the validation. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.

Milk and milk products (or fat coming from milk and milk products), infant formulas, emulsifiers, free fatty acids and other fats- and oils-derived matrices are excluded from the scope of this document.

Table of contents
  • Avant-propos
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Réactifs
  • 6 Appareillage
  • 7 Transport et stockage
  • 8 Mode opératoire
  • 9 Expression des résultats
  • 10 Notes
  • 11 Fidélité
  • Annexe A (normative) Tableaux explicatifs
  • Annexe B (informative) Résultats statistiques de l'étude comparative interlaboratoires ISO
  • Annexe C (informative) Chromatogrammes
  • Bibliographie
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