NF EN ISO 21569
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods
ISO 21569:2005 describes the procedure to qualitatively detect genetically modified organisms (GMOs) and derived products by analysing the nucleic acids extracted from the sample under study. The main focus is on polymerase chain reaction (PCR) based amplification methods. It gives general requirements for the specific detection and identification of target nucleic acid sequences (DNA) and for the confirmation of the identity of the amplified DNA sequence. Guidelines, minimum requirements and performance criteria laid down in ISO 21569:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories. ISO 21569:2005 has been established for food matrices, but could also be applied to other matrices (e.g. feed and plant samples from the environment). Specific examples of methods are provided in Annexes A to D.
ISO 21569:2005 describes the procedure to qualitatively detect genetically modified organisms (GMOs) and derived products by analysing the nucleic acids extracted from the sample under study. The main focus is on polymerase chain reaction (PCR) based amplification methods.
It gives general requirements for the specific detection and identification of target nucleic acid sequences (DNA) and for the confirmation of the identity of the amplified DNA sequence.
Guidelines, minimum requirements and performance criteria laid down in ISO 21569:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories.
ISO 21569:2005 has been established for food matrices, but could also be applied to other matrices (e.g. feed and plant samples from the environment).
Specific examples of methods are provided in Annexes A to D.
Le présent document a pour objectif d'homogénéiser les résultats obtenus dans les laboratoires par la définition d'un cadre général auquel doit satisfaire toute méthode de détection des organismes végétaux génétiquement modifiés (OGM) basée sur les acides nucléiques et plus particulièrement sur la technique PCR (réaction de polymérisation en chaîne).
- Avant-proposiv
- Introductionv
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1 Domaine d'application1
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2 Références normatives1
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3 Termes et définitions1
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4 Principe de la méthode2
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5 Réactifs2
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6 Appareillage et équipements3
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7 Mode opératoire3
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8 Interprétation6
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9 Expression des résultats et assurance qualité6
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10 Rapport d'essai7
- Annexe A (informative) Méthodes spécifiques au taxon8
- Annexe B (informative) Méthodes de criblage25
- Annexe C (informative) Méthodes spécifiques au construit41
- Annexe D (informative) Méthodes spécifiques aux évènements64
- Bibliographie69
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