NF EN ISO 8199

NF EN ISO 8199

November 2018
Standard Current

Water quality - General requirements and guidance for microbiological examinations by culture

This document specifies requirements and gives guidance for performing the manipulations common to each culture technique for the microbiological examination of water, particularly the preparation of samples, culture media, and general apparatus and glassware, unless otherwise required in the specific standard. It also describes the various techniques available for detection and enumeration by culture and the criteria for determining which technique is appropriate. This document is mainly intended for examinations for bacteria, yeasts and moulds, but some aspects are also applicable to bacteriophages, viruses and parasites. It excludes techniques not based on culturing microorganisms, such as polymerase chain reaction (PCR) methods.

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Main informations

Collections

National standards and national normative documents

Publication date

November 2018

Number of pages

68 p.

Reference

NF EN ISO 8199

ICS Codes

07.100.20   Microbiology of water

Classification index

T90-400

Print number

1

European kinship

EN ISO 8199:2018
Sumary
Water quality - General requirements and guidance for microbiological examinations by culture

This document specifies requirements and gives guidance for performing the manipulations common to each culture technique for the microbiological examination of water, particularly the preparation of samples, culture media, and general apparatus and glassware, unless otherwise required in the specific standard. It also describes the various techniques available for detection and enumeration by culture and the criteria for determining which technique is appropriate.

This document is mainly intended for examinations for bacteria, yeasts and moulds, but some aspects are also applicable to bacteriophages, viruses and parasites. It excludes techniques not based on culturing microorganisms, such as polymerase chain reaction (PCR) methods.

Replaced standards (1)
NF EN ISO 8199
January 2008
Standard Cancelled
Water quality - General guidance on the enumeration of micro-organisms by culture

<p>ISO 8199:2005 presents guidance for carrying out manipulations which are common to each technique for the microbiological examination of water, particularly the preparation of samples, culture media and apparatus. It also describes the various enumeration techniques available and the criteria for the choice of a particular technique. It is mainly intended for bacteria, yeasts and moulds. Some aspects are also applicable to viruses and parasites.</p>

Table of contents
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  • Avant-propos
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Exigences générales concernant les mesurages
  • 6 Diluants et milieux de culture
  • 7 Stérilisation et décontamination
  • 8 Échantillons et manipulation des échantillons
  • 9 Méthodes de dénombrement (quantitatives)
  • 10 Méthodes de détection (qualitatives)
  • 11 Caractéristiques de performances des méthodes
  • 12 Contrôle qualité analytique
  • Annexe C (normative) Numération et calculs avec deux boîtes de Petri par dilution
  • Annexe D (normative) Composition, préparation et essai de performances des diluants
  • Annexe A (informative) Critères de choix de la technique
  • Annexe B (informative) Intervalles de confiance pour la technique de numération de colonies et choix de la méthode de calcul dans des cas particuliers
  • Bibliographie
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