NF EN ISO 8199
Water quality - General guidance on the enumeration of micro-organisms by culture
ISO 8199:2005 presents guidance for carrying out manipulations which are common to each technique for the microbiological examination of water, particularly the preparation of samples, culture media and apparatus. It also describes the various enumeration techniques available and the criteria for the choice of a particular technique. It is mainly intended for bacteria, yeasts and moulds. Some aspects are also applicable to viruses and parasites.
ISO 8199:2005 presents guidance for carrying out manipulations which are common to each technique for the microbiological examination of water, particularly the preparation of samples, culture media and apparatus. It also describes the various enumeration techniques available and the criteria for the choice of a particular technique. It is mainly intended for bacteria, yeasts and moulds. Some aspects are also applicable to viruses and parasites.
Le présent fascicule de documentation s'adresse aux laboratoires pratiquant les analyses microbiologiques des eaux. Il expose des directives générales et des recommandations guidant dans la mise en place des bonnes pratiques de laboratoires.
This document specifies requirements and gives guidance for performing the manipulations common to each culture technique for the microbiological examination of water, particularly the preparation of samples, culture media, and general apparatus and glassware, unless otherwise required in the specific standard. It also describes the various techniques available for detection and enumeration by culture and the criteria for determining which technique is appropriate. This document is mainly intended for examinations for bacteria, yeasts and moulds, but some aspects are also applicable to bacteriophages, viruses and parasites. It excludes techniques not based on culturing microorganisms, such as polymerase chain reaction (PCR) methods.
- Avant-proposiv
- Introductionvi
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1 Domaine d'application1
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2 Références normatives1
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3 Principe1
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4 Généralités2
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5 Diluants et milieux de culture2
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6 Stérilisation des appareils et de la verrerie5
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7 Échantillons5
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8 Dénombrement après ensemencement des prises d'essai de l'échantillon sur (ou dans) un milieu solide6
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9 Dénombrement par ensemencement en milieu liquide16
- Annexe A (informative) Critères de choix d'une technique de dénombrement24
- Annexe B (informative) Tables NPP29
- Bibliographie37
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