FD ISO 12890

FD ISO 12890

August 2003
Published document Current

Water quality - Determination of toxicity to embryos and larvae of freshwater fish - Semi-static method

This International Standard specifies a semi-static method for determination of toxicity of chemicals, waters and wastewaters to embryos and early larval developmental stages of a species of freshwater fish, Danio rerio (Hamilton-Buchanan), Teleostei, Cyprinidae (common name zebrafish). Where necessary this determination may include an acute toxicity test using Danio rerio to determine the 96-h LC50 for zebrafish in accordance with ISO 7346-1, ISO 7346-2 or ISO 7346-3. The method is also applicable to freshwater fish other than zebrafish if appropriate modifications of the test conditions are made, particularly with regard to the temperature and volume per fish biomass.

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Main informations

Collections

National standards and national normative documents

Publication date

August 2003

Number of pages

21 p.

Reference

FD ISO 12890

ICS Codes

13.060.10   Water of natural resources
13.060.70   Examination of biological properties of water

Classification index

T90-331

Print number

1 - 23/09/2003

International kinship

ISO 12890:1999
Sumary
Water quality - Determination of toxicity to embryos and larvae of freshwater fish - Semi-static method

This International Standard specifies a semi-static method for determination of toxicity of chemicals, waters and

wastewaters to embryos and early larval developmental stages of a species of freshwater fish, Danio rerio

(Hamilton-Buchanan), Teleostei, Cyprinidae (common name zebrafish). Where necessary this determination may

include an acute toxicity test using Danio rerio to determine the 96-h LC50 for zebrafish in accordance with

ISO 7346-1, ISO 7346-2 or ISO 7346-3.

The method is also applicable to freshwater fish other than zebrafish if appropriate modifications of the test

conditions are made, particularly with regard to the temperature and volume per fish biomass.

Table of contents
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  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    2
  • 4 Principe
    2
  • 5 Environnement d'essai
    3
  • 6 Poisson d'essai
    3
  • 7 Réactifs
    3
  • 8 Appareillage
    4
  • 9 Mode opératoire
    5
  • 10 Résultats
    6
  • 11 Rapport d'essai
    7
  • Annexe A (informative) Conditions de ponte des neufs du poisson-zèbre
    9
  • Annexe B (informative) Exemples de données relatives à la mortalité initiale et aux temps médians pour l'éclosion et le taux de survie
    11
  • Bibliographie
    14
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