ISO 21528-1:2017
Microbiology of the food chain - Horizontal methods for the detection and enumeration of enterobacteriaceae - Part 1 : detection of enterobacteriaceae
ISO 21528-1:2017 specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to- products intended for human consumption and the feeding of animals, and- environmental samples in the area of primary production, food production and food handling.This method is applicable- when the microorganisms sought are expected to need resuscitation by enrichment, and- when the number sought is expected to be below 100 per millilitre or per gram of test sample.A limitation on the applicability of ISO 21528-1:2017 is imposed by the susceptibility of the method to a large degree of variability (see Clause 11).NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See Annex A.
ISO 21528-1:2017 specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to
- products intended for human consumption and the feeding of animals, and
- environmental samples in the area of primary production, food production and food handling.
This method is applicable
- when the microorganisms sought are expected to need resuscitation by enrichment, and
- when the number sought is expected to be below 100 per millilitre or per gram of test sample.
A limitation on the applicability of ISO 21528-1:2017 is imposed by the susceptibility of the method to a large degree of variability (see Clause 11).
NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See Annex A.
ISO 21528-1:2004 specifies a method, with pre-enrichment, for the detection of Enterobacteriaceae. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling. Enumeration is carried out by calculation of the most probable number (MPN) after incubation at 37 °C in liquid medium. This method is applicable when the microorganisms sought are expected to need resuscitation before enrichment, and when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample. A limitation on the applicability of ISO 21528-1:2004 is imposed by the susceptibility of the method to a large degree of variability
The Requirements department helps you quickly locate within the normative text:
- mandatory clauses to satisfy,
- non-essential but useful clauses to know, such as permissions and recommendations.
The identification of these types of clauses is based on the document “ISO / IEC Directives, Part 2 - Principles and rules of structure and drafting of ISO documents ”as well as on a constantly enriched list of verbal forms.
With Requirements, quickly access the main part of the normative text!

At a glance, you will be able to identify the additions, deletions or modifications to a text, table, figure and formula.

The Redlines + service is offered to you on the collection of French standards in force, in French language and in HTML and PDF format.
For an overview of the service, click on View a standard in redline format
COBAZ is the simple and effective solution to meet the normative needs related to your activity, in France and abroad.
Available by subscription, CObaz is THE modular solution to compose according to your needs today and tomorrow. Quickly discover CObaz!
Request your free, no-obligation live demo
I discover COBAZ