This document provides a list of target sequences that can be used to screen for the presence of genetically modified (GM) material in cotton and cotton products. This document is applicable to cottonseed, cotton leaf, cotton fibre and cotton fibre-derived materials from which sufficiently high-quality PCR amplifiable DNA can be extracted. Methods describing the extraction of DNA from different cotton samples can be found in ISO 5354-1[1]. NOTE 1 The list of target sequences provides guidance for the screening of all currently known GM cotton events and GM cotton events that contain the same DNA sequences. Further guidance on screening of foodstuffs is provided in CEN/TS 16707[2]. NOTE 2 Sampling is outside of the scope of this document. Information on sampling cotton products can be found in ISO 1130:1975[3] and in ASTM D1441-12[4].
This document provides a list of target sequences that can be used to screen for the presence of genetically modified (GM) material in cotton and cotton products.
This document is applicable to cottonseed, cotton leaf, cotton fibre and cotton fibre-derived materials from which sufficiently high-quality PCR amplifiable DNA can be extracted.
Methods describing the extraction of DNA from different cotton samples can be found in ISO 5354-1[1].
NOTE 1 The list of target sequences provides guidance for the screening of all currently known GM cotton events and GM cotton events that contain the same DNA sequences. Further guidance on screening of foodstuffs is provided in CEN/TS 16707[2].
NOTE 2 Sampling is outside of the scope of this document. Information on sampling cotton products can be found in ISO 1130:1975[3] and in ASTM D1441-12[4].
<p>This document provides requirements and recommendations to laboratories that perform genetically modified organism (GMO) analyses in cottonseed, leaf, cotton fibre and cotton fibre-derived materials.</p> <p>The following are within the scope of this document:</p> <p>a) identifying the materials to be assessed, based on the probability of obtaining good quality, fit for purpose DNA from the materials in subsequent steps in the cotton cloth production process;</p> <p>b) specifying a method for efficient DNA isolation from cotton and cotton-derived materials described under point a);</p> <p>c) specifying the cotton-specific method(s) to be used as control for amplifiable DNA;</p> <p>d) specifying the screening procedure that provides optimal chances to detect GMOs as a result of the performance of the lowest number of genetically modified (GM) element screening assays. </p> <p>NOTE 1 The protocol allows for the screening of all currently known GM cotton events and is set up in a way that optimizes the probability of also detecting unknown GM cotton events that possibly contain similar DNA sequences. Further information is given in CEN/TS 16707.</p> <p>Sampling is outside of the scope of this document. </p> <p>NOTE 2 A recommended sampling method is given in ISO 6497. General guidance for the sampling of bulk materials or for cotton-based products is available in standards such as ASTM D1441‑12 and CEN/TS 15568.</p>
<p>This document provides requirements and recommendations to laboratories that perform genetically modified organism (GMO) analyses in cottonseed, leaf, cotton fibre and cotton fibre-derived materials.</p> <p>The following are within the scope of this document:</p> <p>a) identifying the materials to be assessed, based on the probability of obtaining good quality, fit for purpose DNA from the materials in subsequent steps in the cotton cloth production process;</p> <p>b) specifying a method for efficient DNA isolation from cotton and cotton-derived materials described under point a);</p> <p>c) specifying the cotton-specific method(s) to be used as control for amplifiable DNA;</p> <p>d) specifying the screening procedure that provides optimal chances to detect GMOs as a result of the performance of the lowest number of genetically modified (GM) element screening assays. </p> <p>NOTE 1 The protocol allows for the screening of all currently known GM cotton events and is set up in a way that optimizes the probability of also detecting unknown GM cotton events that possibly contain similar DNA sequences. Further information is given in CEN/TS 16707.</p> <p>Sampling is outside of the scope of this document. </p> <p>NOTE 2 A recommended sampling method is given in ISO 6497. General guidance for the sampling of bulk materials or for cotton-based products is available in standards such as ASTM D1441‑12 and CEN/TS 15568.</p>
COBAZ is the simple and effective solution to meet the normative needs related to your activity, in France and abroad.
Available by subscription, CObaz is THE modular solution to compose according to your needs today and tomorrow. Quickly discover CObaz!
Request your free, no-obligation live demo
I discover COBAZ