NF EN ISO 10253

NF EN ISO 10253

June 2006
Standard Cancelled

Water quality - Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum

ISO 10253:2006 specifies a method for the determination of the inhibition of growth of the unicellular marine algae Skeletonema costatum and Phaeodactylum tricornutum by substances and mixtures contained in sea water.The method can be used for testing substances that are readily soluble in water and are not significantly degraded or eliminated in any other way from the test medium.

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Main informations

Collections

National standards and national normative documents

Publication date

June 2006

Number of pages

18 p.

Reference

NF EN ISO 10253

ICS Codes

13.060.70   Examination of biological properties of water

Classification index

T90-311

Print number

1 - 13/06/2006

International kinship

ISO 10253:2006

European kinship

EN ISO 10253:2006
Sumary
Water quality - Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum

ISO 10253:2006 specifies a method for the determination of the inhibition of growth of the unicellular marine algae Skeletonema costatum and Phaeodactylum tricornutum by substances and mixtures contained in sea water.The method can be used for testing substances that are readily soluble in water and are not significantly degraded or eliminated in any other way from the test medium.

Replaced standards (1)
NF EN ISO 10253
April 1998
Standard Cancelled
Water quality. Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum.

Standard replaced by (1)
NF EN ISO 10253
December 2016
Standard Current
Water quality - Marine algal growth inhibition test with Skeletonema sp. and Phaeodactylum tricornutum

ISO 10253:2016 specifies a method for the determination of the inhibition of growth of the unicellular marine algae Skeletonema sp. and Phaeodactylum tricornutum by substances and mixtures contained in sea water or by environmental water samples (effluents, elutriates, etc.). The method can be used for testing substances that are readily soluble in water and are not significantly degraded or eliminated in any other way from the test medium.

Table of contents
  • Avant-propos
    iv
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    1
  • 4 Principe
    2
  • 5 Matériels
    2
  • 5.1 Organismes d'essai
    2
  • 5.2 Eau
    3
  • 5.3 Eau de mer
    3
  • 5.4 Nutriments
    4
  • 6 Appareillage
    5
  • 7 Mode opératoire
    5
  • 7.1 Préparation du milieu de croissance
    5
  • 7.2 Préparation de la préculture et de l'inoculum
    5
  • 7.3 Sélection des concentrations de l'essai
    6
  • 7.4 Préparation des solutions mères de substance d'essai
    6
  • 7.5 Préparation des lots d'essai et des témoins
    6
  • 7.6 Incubation
    7
  • 7.7 Mesurages
    7
  • 8 Critères de validité
    7
  • 9 Interprétation des données
    8
  • 9.1 Tracé des courbes de croissance
    8
  • 9.2 Calcul du pourcentage d'inhibition
    8
  • 9.3 Détermination des valeurs de EC(r)x
    9
  • 10 Expression des résultats
    9
  • 11 Interprétation des résultats
    9
  • 12 Reproductibilité
    9
  • 13 Rapport d'essai
    10
  • Annexe A (informative) Préparation des gammes de dilution des mélanges en eau de mer (effluents ou élutriats)
    11
  • Bibliographie
    12
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