NF EN ISO 10634

Le présent fascicule de documentation s'adresse aux laboratoires réalisant des essais écotoxicologiques dans le but d'évaluer les risques des produits chimiques pour l'homme et l'environnement. Il expose des directives générales et des recommandations pour la mise en oeuvre d'essais de biodégradabilité sur les composés organiques faiblement solubles dans l'eau.

This document specifies techniques for preparing poorly water-soluble organic compounds (i.e. liquid and solid compounds) with a solubility in water of less than approximately 100 mg/l and introducing them into test vessels for a subsequent biodegradability test in an aqueous medium using standard methods. The subsequent tests on biodegradability are primarily methods using the analysis of the released carbon dioxide described in ISO 9439 and the determination of the oxygen described in ISO 9408 and following the usual precautions for ISO 10707. Thus, one can notice that the methods measuring the removal of dissolved organic carbon (DOC) are not appropriate. This document does not specify the biodegradation test methods. It is restricted to describing techniques for introducing the test compounds into the test medium and to keeping them in a dispersed state[4]. These techniques are implemented while observing the experimental conditions described in the standardized methods for evaluating biodegradability. ISO 9439, based on CO2 evolution, is not suitable for testing volatile compounds. Some of the preparation methods described in this document might not be accepted by regulators for making conclusions on the ready biodegradability of tested compounds. Examples of biodegradability curves are given in Annex A.