NF EN ISO 11731

NF EN ISO 11731

July 2017
Standard Current

Water quality - Enumeration of Legionella

ISO 11731:2017 specifies culture methods for the isolation of Legionella and estimation of their numbers in water samples.These methods are applicable to all kinds of water samples including potable, industrial, waste and natural waters. These methods can be used for water related matrices, e.g. biofilms, sediments, etc.Not all Legionella species are culturable; therefore, the methods described in this document do not recover all species of Legionella.

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Main informations

Collections

National standards and national normative documents

Publication date

July 2017

Number of pages

46 p.

Reference

NF EN ISO 11731

ICS Codes

07.100.20   Microbiology of water

Classification index

T90-430

Print number

1

International kinship

ISO 11731:2017

European kinship

EN ISO 11731:2017
Sumary
Water quality - Enumeration of Legionella

ISO 11731:2017 specifies culture methods for the isolation of Legionella and estimation of their numbers in water samples.

These methods are applicable to all kinds of water samples including potable, industrial, waste and natural waters. These methods can be used for water related matrices, e.g. biofilms, sediments, etc.

Not all Legionella species are culturable; therefore, the methods described in this document do not recover all species of Legionella.

Replaced standards (1)
NF EN ISO 11731-2
July 2008
Standard Cancelled
Water quality - Detection and enumeration of Legionella - Part 2 : direct membrane filtration method for waters with low bacterial counts

ISO 11731-2:2004 describes a monitoring method for the isolation and enumeration of Legionella organisms in water intended for human use (e.g. hot and cold water, water used for washing), for human consumption and for treated bathing waters (e.g. swimming pools). It is especially suitable for waters with prospected low numbers of Legionella. As the growth of Legionella may be inhibited by overgrowth of other bacterial colonies on the membrane the method is only suitable for waters containing low bacterial.

Table of contents
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Appareillage et verrerie
  • 6 Milieux de culture et réactifs
  • 7 Échantillonnage
  • 8 Mode opératoire
  • 9 Expression des résultats
  • 10 Rapport d'essai
  • 11 Assurance qualité
  • Annexe A Espèces de Legionella
  • Annexe B Milieux de culture
  • Annexe C Diluants
  • Annexe D Solution d'acide
  • Annexe E Retrait des bactéries par grattage ou frottement des membranes filtrantes
  • Annexe F Technique de centrifugation
  • Annexe G Technique des anticorps par immunofluorescence indirecte pour l'identification des espèces de Legionella
  • Annexe H Données de performance
  • Annexe I Matrices de pré-traitement liées à l'eau
  • Annexe J Matrice de décision
  • Bibliographie
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