NF EN ISO 11816-1

NF EN ISO 11816-1

December 2013
Standard Current

Milk and milk products - Determination of alkaline phosphatase activity - Part 1 : fluorimetric method for milk and milk-based drinks

ISO 11816-1|IDF 155-1:2013 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks. This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution.The instrument can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with alkaline phosphatase-free milk so as to obtain a measurement not higher than 7 000 mU/l.

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Main informations

Collections

National standards and national normative documents

Publication date

December 2013

Number of pages

23 p.

Reference

NF EN ISO 11816-1

ICS Codes

67.100.10   Milk and processed milk products

Classification index

V04-054-1

Print number

1 - 12/12/2013

International kinship

European kinship

EN ISO 11816-1:2013
Sumary
Milk and milk products - Determination of alkaline phosphatase activity - Part 1 : fluorimetric method for milk and milk-based drinks

ISO 11816-1|IDF 155-1:2013 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks. This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution.

The instrument can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with alkaline phosphatase-free milk so as to obtain a measurement not higher than 7 000 mU/l.

Replaced standards (1)
NF EN ISO 11816-1
July 2006
Standard Cancelled
Milk and milk products - Determination of alkaline phosphatase activity - Part 1 : fluorimetric method for milk and milk-based drinks

ISO 11816-1|IDF 155-1:2006 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in pasteurised whole milk, semi-skimmed milk, skimmed milk and flavoured milks. The method is applicable for milk from cows, sheep and goats, and milk-based drinks. The method is also suitable for the determination of high alkaline phosphatase activity in raw milk and heat-treated milk with activities of more than 2 000 mU/l after dilution of the sample as specified.

Table of contents
  • Avant-propos
    v
  • 1 Domaine d'application
    1
  • 2 Termes et définitions
    1
  • 3 Principe
    1
  • 4 Réactifs
    2
  • 5 Appareillage
    3
  • 6 Échantillonnage
    3
  • 7 Préparations
    3
  • 7.1 Lait exempt de phosphatase alcaline
    3
  • 7.2 Préparation de l'échantillon pour essai
    4
  • 8 Mode opératoire
    4
  • 8.1 Vérification des performances de l'appareillage
    4
  • 8.2 Contrôles de réactifs pour évaluer l'adéquation du substrat de travail prêt à l'emploi
    5
  • 8.3 Étalonnage
    5
  • 8.4 Détermination
    6
  • 8.5 Contrôles relatifs aux échantillons pour essai
    7
  • 9 Calcul et expression des résultats
    7
  • 9.1 Rapport d'étalonnage
    7
  • 9.2 Calcul
    8
  • 9.3 Expression des résultats d'essai
    8
  • 10 Fidélité
    8
  • 10.1 Essai interlaboratoires
    8
  • 10.2 Répétabilité
    9
  • 10.3 Reproductibilité
    9
  • 11 Rapport d'essai
    9
  • Annexe A (informative) Essais interlaboratoires
    10
  • Bibliographie
    13
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