NF EN ISO 11816-1
Milk and milk products - Determination of alkaline phosphatase activity - Part 1 : fluorimetric method for milk and milk-based drinks
ISO 11816-1|IDF 155-1:2013 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks. This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution.The instrument can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with alkaline phosphatase-free milk so as to obtain a measurement not higher than 7 000 mU/l.
ISO 11816-1|IDF 155-1:2013 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks. This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution.
The instrument can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with alkaline phosphatase-free milk so as to obtain a measurement not higher than 7 000 mU/l.
ISO 11816-1|IDF 155-1:2006 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in pasteurised whole milk, semi-skimmed milk, skimmed milk and flavoured milks. The method is applicable for milk from cows, sheep and goats, and milk-based drinks. The method is also suitable for the determination of high alkaline phosphatase activity in raw milk and heat-treated milk with activities of more than 2 000 mU/l after dilution of the sample as specified.
- Avant-proposv
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1 Domaine d'application1
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2 Termes et définitions1
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3 Principe1
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4 Réactifs2
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5 Appareillage3
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6 Échantillonnage3
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7 Préparations3
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7.1 Lait exempt de phosphatase alcaline3
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7.2 Préparation de l'échantillon pour essai4
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8 Mode opératoire4
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8.1 Vérification des performances de l'appareillage4
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8.2 Contrôles de réactifs pour évaluer l'adéquation du substrat de travail prêt à l'emploi5
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8.3 Étalonnage5
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8.4 Détermination6
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8.5 Contrôles relatifs aux échantillons pour essai7
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9 Calcul et expression des résultats7
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9.1 Rapport d'étalonnage7
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9.2 Calcul8
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9.3 Expression des résultats d'essai8
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10 Fidélité8
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10.1 Essai interlaboratoires8
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10.2 Répétabilité9
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10.3 Reproductibilité9
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11 Rapport d'essai9
- Annexe A (informative) Essais interlaboratoires10
- Bibliographie13
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