NF EN ISO 11816-1

NF EN ISO 11816-1

July 2006
Standard Cancelled

Milk and milk products - Determination of alkaline phosphatase activity - Part 1 : fluorimetric method for milk and milk-based drinks

ISO 11816-1|IDF 155-1:2006 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in pasteurised whole milk, semi-skimmed milk, skimmed milk and flavoured milks. The method is applicable for milk from cows, sheep and goats, and milk-based drinks. The method is also suitable for the determination of high alkaline phosphatase activity in raw milk and heat-treated milk with activities of more than 2 000 mU/l after dilution of the sample as specified.

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Collections

National standards and national normative documents

Publication date

July 2006

Number of pages

20 p.

Reference

NF EN ISO 11816-1

ICS Codes

67.100.10   Milk and processed milk products

Classification index

V04-054-1

Print number

1 - 18/07/2006

International kinship

ISO 11816-1:2006

European kinship

EN ISO 11816-1:2006
Sumary
Milk and milk products - Determination of alkaline phosphatase activity - Part 1 : fluorimetric method for milk and milk-based drinks

ISO 11816-1|IDF 155-1:2006 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in pasteurised whole milk, semi-skimmed milk, skimmed milk and flavoured milks. The method is applicable for milk from cows, sheep and goats, and milk-based drinks.

The method is also suitable for the determination of high alkaline phosphatase activity in raw milk and heat-treated milk with activities of more than 2 000 mU/l after dilution of the sample as specified.

Replaced standards (1)
NF EN ISO 11816-1
June 2000
Standard Cancelled
Milk and milk products - Determination of alkaline phosphatase activity using a fluorimetric method - Part 1 : milk and milk-based drinks

Standard replaced by (1)
NF EN ISO 11816-1
December 2013
Standard Current
Milk and milk products - Determination of alkaline phosphatase activity - Part 1 : fluorimetric method for milk and milk-based drinks

ISO 11816-1|IDF 155-1:2013 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks. This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution. The instrument can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with alkaline phosphatase-free milk so as to obtain a measurement not higher than 7 000 mU/l.

Table of contents
  • Avant-propos
    iv
  • 1
    Domaine d'application 1
  • 2
    Termes et définitions 1
  • 3 Principe
    1
  • 4
    Réactifs 2
  • 5
    Appareillage 3
  • 6
    Échantillonnage 3
  • 7 Préparations
    3
  • 7.1
    Lait exempt de phosphatase alcaline 3
  • 7.2
    Préparation de l'échantillon pour essai 4
  • 8
    Mode opératoire 4
  • 8.1
    Vérification des performances de l'appareillage 4
  • 8.2 Étalonnage
    4
  • 8.3 Détermination
    5
  • 8.4
    Essais de contrôle 5
  • 9
    Calcul et expression des résultats 7
  • 9.1
    Ratio d'étalonnage 7
  • 9.2 Calcul
    7
  • 9.3
    Expression des résultats des essais 8
  • 10
    Fidélité 8
  • 10.1
    Essai interlaboratoires 8
  • 10.2 Répétabilité
    8
  • 10.3 Reproductibilité
    8
  • 11
    Rapport d'essai 9
  • Annexe A (informative) Essai interlaboratoires
    10
  • Bibliographie
    12
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