NF EN ISO 12966-4

NF EN ISO 12966-4

August 2015
Standard Current

Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 4 : determination by capillary gas chromatography

ISO 12966-4:2015 specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC). Fatty acid methyl esters from C8 to C24 can be separated using this part of ISO 12966 including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters.The method is applicable to crude, refined, partially hydrogenated, or fully hydrogenated fats, oils, and fatty acids derived from animal and vegetable sources.This method is not suitable for the analysis of dairy, ruminant fats and oils, or products supplemented with conjugated linoleic acid (CLA). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 12966.ISO 12966-4:2015 is not applicable to di-, tri-, polymerized and oxidized fatty acids, and fats and oils.

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National standards and national normative documents

Publication date

August 2015

Number of pages

30 p.

Reference

NF EN ISO 12966-4

ICS Codes

67.200.10   Animal and vegetable fats and oils

Classification index

T60-233-4

Print number

1

International kinship

ISO 12966-4:2015

European kinship

EN ISO 12966-4:2015
Sumary
Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 4 : determination by capillary gas chromatography

ISO 12966-4:2015 specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC). Fatty acid methyl esters from C8 to C24 can be separated using this part of ISO 12966 including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters.

The method is applicable to crude, refined, partially hydrogenated, or fully hydrogenated fats, oils, and fatty acids derived from animal and vegetable sources.

This method is not suitable for the analysis of dairy, ruminant fats and oils, or products supplemented with conjugated linoleic acid (CLA). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 12966.

ISO 12966-4:2015 is not applicable to di-, tri-, polymerized and oxidized fatty acids, and fats and oils.

Replaced standards (2)
NF EN ISO 5508
June 1995
Standard Cancelled
Animal and vegetable fats and oils. Analysis by gas chromatography of methyl esters of fatty acids.

NF EN ISO 15304
May 2002
Standard Cancelled
Animal and vegetable fats and oils - Determination of the content of trans fatty acid isomers of vegetable fats and oils - Gas chromatographic method

Le présent document spécifie une méthode de détermination de la teneur en isomères trans d'acides gras de corps gras d'origine végétale par chromatographie en phase gazeuse utilisant des colonnes capillaires. Cette méthode est particulièrement conçue pour évaluer la teneur en isomères trans obtenus lors du raffinage ou de l'hydrogénation de ces corps gras.

Table of contents
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Principe
  • 4 Réactifs et matériaux
  • 5 Appareillage
  • 6 Échantillonnage
  • 7 Préparation de l'échantillon pour essai
  • 8 Préparation des esters méthyliques de corps gras et d'acides gras
  • 9 Mode opératoire
  • 10 Calculs
  • 11 Fidélité
  • 12 Rapport d'essai
  • Annexe A Facteur de correction théorique (FCT) du détecteur à ionisation de flamme pour les esters méthyliques d'acides gras (EMAG)
  • Annexe B Exemples de chromatogrammes
  • Annexe C Comparaison de la composition en EMAG avec deux colonnes CPG différentes
  • Annexe D Résultats d'un essai interlaboratoires
  • Bibliographie
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