NF EN ISO 12966-4

NF EN ISO 12966-4

August 2015
Standard Current

Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 4 : determination by capillary gas chromatography

ISO 12966-4:2015 specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC). Fatty acid methyl esters from C8 to C24 can be separated using this part of ISO 12966 including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters.The method is applicable to crude, refined, partially hydrogenated, or fully hydrogenated fats, oils, and fatty acids derived from animal and vegetable sources.This method is not suitable for the analysis of dairy, ruminant fats and oils, or products supplemented with conjugated linoleic acid (CLA). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 12966.ISO 12966-4:2015 is not applicable to di-, tri-, polymerized and oxidized fatty acids, and fats and oils.

See more
View the extract
Main informations

Collections

National standards and national normative documents

Publication date

August 2015

Number of pages

27 p.

Reference

NF EN ISO 12966-4

ICS Codes

67.200.10   Animal and vegetable fats and oils

Classification index

T60-233-4

Print number

1

International kinship

ISO 12966-4:2015

European kinship

EN ISO 12966-4:2015
Sumary
Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 4 : determination by capillary gas chromatography

ISO 12966-4:2015 specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC). Fatty acid methyl esters from C8 to C24 can be separated using this part of ISO 12966 including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters.

The method is applicable to crude, refined, partially hydrogenated, or fully hydrogenated fats, oils, and fatty acids derived from animal and vegetable sources.

This method is not suitable for the analysis of dairy, ruminant fats and oils, or products supplemented with conjugated linoleic acid (CLA). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 12966.

ISO 12966-4:2015 is not applicable to di-, tri-, polymerized and oxidized fatty acids, and fats and oils.

Replaced standards (2)
NF EN ISO 5508
June 1995
Standard Cancelled
Animal and vegetable fats and oils. Analysis by gas chromatography of methyl esters of fatty acids.

NF EN ISO 15304
May 2002
Standard Cancelled
Animal and vegetable fats and oils - Determination of the content of trans fatty acid isomers of vegetable fats and oils - Gas chromatographic method

This International Standard specifies a gas chromatographic method using capillary columns for the determination of the content of trans fatty acid isomers of vegetable oils and fats. The method is specially designed to evaluate, by a single capillary gas chromatographic (GC) procedure, the level of trans isomers as formed during (high temperature) refining, or during hydrogenation of vegetable oils or fats. The method may also be used to report all other fatty acids (e.g. to obtain the full fatty acid composition and total amounts of saturated fatty acids, mono-unsaturated fatty acids and poly-unsaturated fatty acids) from the same sample and same analysis.

Table of contents
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Principe
  • 4 Réactifs et matériaux
  • 5 Appareillage
  • 6 Échantillonnage
  • 7 Préparation de l'échantillon pour essai
  • 8 Préparation des esters méthyliques de corps gras et d'acides gras
  • 9 Mode opératoire
  • 10 Calculs
  • 11 Fidélité
  • 12 Rapport d'essai
  • Annexe A Facteur de correction théorique (FCT) du détecteur à ionisation de flamme pour les esters méthyliques d'acides gras (EMAG)
  • Annexe B Exemples de chromatogrammes
  • Annexe C Comparaison de la composition en EMAG avec deux colonnes CPG différentes
  • Annexe D Résultats d'un essai interlaboratoires
  • Bibliographie
ZOOM ON ... the Requirements department
To comply with a standard, you need to quickly understand its issues in order to determine its impact on your activity.

The Requirements department helps you quickly locate within the normative text:
- mandatory clauses to satisfy,
- non-essential but useful clauses to know, such as permissions and recommendations.

The identification of these types of clauses is based on the document “ISO / IEC Directives, Part 2 - Principles and rules of structure and drafting of ISO documents ”as well as on a constantly enriched list of verbal forms.

With Requirements, quickly access the main part of the normative text!

With Requirements, quickly access the main part of the normative text!
New: UPSELL service
- The UPSELL service allows you to easily update one of your standards.
- With a single click, add a new language, the Requirements or Redline+ service and add one or more additional users.
- Whether you are in the process of acquiring a standard or it is already available in your personal space, the UPSELL service is available at every stage to help you understand it and implement it within your organization.
Need to identify, monitor and decipher standards?

COBAZ is the simple and effective solution to meet the normative needs related to your activity, in France and abroad.

Available by subscription, CObaz is THE modular solution to compose according to your needs today and tomorrow. Quickly discover CObaz!

Request your free, no-obligation live demo

I discover COBAZ