NF EN ISO 13903

NF EN ISO 13903

November 2005
Standard Current

Animal feeding stuffs - Determination of amino acids content

ISO 13903:2005 describes the determination of free (synthetic and natural) and total (peptide-bound and free) amino acids in feeding stuffs, using an amino acid analyser or HPLC equipment. It is applicable to the following amino acids: sum of cystine and cysteine; methionine; lysine; threonine; alanine; arginine; aspartic acid; glutamic acid; glycine; histidine; isoleucine; leucine; phenylalanine; proline; serine; tyrosine; valine.The method does not distinguish between the salts of amino acids, nor does it differentiate between D and L forms of amino acids. It is not valid for the determination of tryptophan or hydroxy analogues of amino acids.Limits of quantification depend on the chromatographic equipment, but levels as low as: 0,3 g/kg total lysine; 0,25 g/kg total methionine; 0,35 g/kg total cystine plus cysteine; 0,2 g/kg total threonine; 0,035g/kg free lysine; 0,035g/kg free methionine; and 0,03g/kg free threonine can typically be analysed.

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Main informations

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National standards and national normative documents

Publication date

November 2005

Number of pages

25 p.

Reference

NF EN ISO 13903

ICS Codes

65.120   Animal feeding stuffs

Classification index

V18-113

Print number

1 - 13/10/2005

International kinship

ISO 13903:2005

European kinship

EN ISO 13903:2005
Sumary
Animal feeding stuffs - Determination of amino acids content

ISO 13903:2005 describes the determination of free (synthetic and natural) and total (peptide-bound and free) amino acids in feeding stuffs, using an amino acid analyser or HPLC equipment. It is applicable to the following amino acids: sum of cystine and cysteine; methionine; lysine; threonine; alanine; arginine; aspartic acid; glutamic acid; glycine; histidine; isoleucine; leucine; phenylalanine; proline; serine; tyrosine; valine.

The method does not distinguish between the salts of amino acids, nor does it differentiate between D and L forms of amino acids. It is not valid for the determination of tryptophan or hydroxy analogues of amino acids.

Limits of quantification depend on the chromatographic equipment, but levels as low as: 0,3 g/kg total lysine; 0,25 g/kg total methionine; 0,35 g/kg total cystine plus cysteine; 0,2 g/kg total threonine; 0,035g/kg free lysine; 0,035g/kg free methionine; and 0,03g/kg free threonine can typically be analysed.

Replaced standards (1)
XP V18-113
January 1998
Standard Cancelled
Animal feeding stuffs. Determination of amino acids.

Le présent document prescrit une méthode d'analyse générale pour le dosage des acides aminés communs, à l'exception du tryptophane. Cette méthode ne permet pas de différencier les formes D et L, ni les sels d'acides aminés, ni de déterminer les analogues hydroxylés de ces acides aminés.

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