NF EN ISO 19020

NF EN ISO 19020

August 2017
Standard Current

Microbiology of the food chain - Horizontal method for the immunoenzymatic detection of staphylococcal enterotoxins in foodstuffs

ISO 19020:2017 specifies a screening method for the detection of staphylococcal enterotoxins SEA, SEB, SECs, SED and SEE in foodstuffs. It consists of two main steps: a) extraction followed by a concentration based on dialysis principle; and b) an immunoenzymatic detection using commercially available detection kits.ISO 19020:2017 is applicable to the screening of staphylococcal enterotoxins SEA to SEE in products intended for human consumption.Other staphylococcal enterotoxins such as types SEG, SEH, SEI, SER, SES and SET can also cause illness. Due to the lack of commercially available detection kits, ISO 19020:2017 is applicable only to types SEA to SEE, but may apply to other types of toxins, subject to validation of the method.

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Main informations

Collections

National standards and national normative documents

Publication date

August 2017

Number of pages

32 p.

Reference

NF EN ISO 19020

ICS Codes

07.100.30   Food microbiology

Classification index

V08-755

Print number

1

International kinship

ISO 19020:2017

European kinship

EN ISO 19020:2017
Sumary
Microbiology of the food chain - Horizontal method for the immunoenzymatic detection of staphylococcal enterotoxins in foodstuffs

ISO 19020:2017 specifies a screening method for the detection of staphylococcal enterotoxins SEA, SEB, SECs, SED and SEE in foodstuffs. It consists of two main steps: a) extraction followed by a concentration based on dialysis principle; and b) an immunoenzymatic detection using commercially available detection kits.

ISO 19020:2017 is applicable to the screening of staphylococcal enterotoxins SEA to SEE in products intended for human consumption.

Other staphylococcal enterotoxins such as types SEG, SEH, SEI, SER, SES and SET can also cause illness. Due to the lack of commercially available detection kits, ISO 19020:2017 is applicable only to types SEA to SEE, but may apply to other types of toxins, subject to validation of the method.

Table of contents
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Réactifs
  • 6 Matériel
  • 7 Échantillonnage
  • 8 Mode opératoire
  • 9 Contrôle de la qualité
  • 10 Expression des résultats
  • 11 Confirmation
  • 12 Caractéristiques de performance de la méthode
  • 13 Rapport d'essai
  • Annexe A Résultats des études interlaboratoires:
    2013
  • Annexe B Résultats des études interlaboratoires:
    2014
  • Annexe C Note concernant les interférences
  • Bibliographie
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