NF EN ISO 20130
Soil quality - Measurement of enzyme activity patterns in soil samples using colorimetric substrates in micro-well plates
This document specifies a method for the measurement of several hydrolase activities (arylamidase, arylsulfatase, β-galactosidase, α-glucosidase, β-glucosidase, N-acetyl-glucosaminidase, acid, alkaline and global phosphatases, urease) simultaneously (or not) in soil samples, using colorimetric substrates. Enzyme activities of soil vary seasonally and depend on soil chemical, physical and biological characteristics. This method can be applied either to detect harmful effects on soil enzyme activities derived from toxic substances or other anthropogenic agents in contaminated soils against a control soil, or to test chemicals.
This document specifies a method for the measurement of several hydrolase activities (arylamidase, arylsulfatase, β-galactosidase, α-glucosidase, β-glucosidase, N-acetyl-glucosaminidase, acid, alkaline and global phosphatases, urease) simultaneously (or not) in soil samples, using colorimetric substrates. Enzyme activities of soil vary seasonally and depend on soil chemical, physical and biological characteristics. This method can be applied either to detect harmful effects on soil enzyme activities derived from toxic substances or other anthropogenic agents in contaminated soils against a control soil, or to test chemicals.
<p>This document specifies a method for the measurement of several hydrolase activities (arylamidase, arylsulfatase, <i>β</i>-galactosidase, <i>α</i>-glucosidase, <i>β</i>-glucosidase, N-acetyl-glucosaminidase, acid, alkaline and global phosphatases, urease) simultaneously (or not) in soil samples, using colorimetric substrates. Enzyme activities of soil vary seasonally and depend on soil chemical, physical and biological characteristics. This method can be applied either to detect harmful effects on soil enzyme activities derived from toxic substances or other anthropogenic agents in contaminated soils against a control soil, or to test chemicals.</p>
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1 Domaine d'application
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2 Références normatives
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3 Termes et définitions, symboles et termes abrégés
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4 Principe
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5 Réactifs
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6 Appareillage et matériaux
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7 Mode opératoire
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8 Calcul des résultats
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9 Expression des résultats
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10 Critères de validité
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11 Validation interlaboratoires
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12 Rapport d'essai
- Annexe A Validation de la méthode et essais intralaboratoire pour l'évaluation des activités enzymatiques du sol par une méthode colorimétrique
- Annexe B Essai interlaboratoires international pour l'évaluation des activités enzymatiques du sol par une méthode colorimétrique
- Bibliographie
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