NF EN ISO 20743
Textiles - Determination of antibacterial activity of textile products
ISO 20743:2013 specifies quantitative test methods to determine the antibacterial activity of all antibacterial textile products including nonwovens. ISO 20743:2013 is applicable to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnishings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting). Based on the intended application and on the environment in which the textile product is to be used and also on the surface properties of the textile properties, the user can select the most suitable of the following three inoculation methods on determination of antibacterial activity: a) absorption method (an evaluation method in which the test bacterial suspension is inoculated directly onto specimens); b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens); c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens). The colony plate count method and the ATP (ATP = Adenosine Tri-phosphate) luminescence method are also specified for measuring the enumeration of bacteria.
ISO 20743:2013 specifies quantitative test methods to determine the antibacterial activity of all antibacterial textile products including nonwovens.
ISO 20743:2013 is applicable to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnishings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).
Based on the intended application and on the environment in which the textile product is to be used and also on the surface properties of the textile properties, the user can select the most suitable of the following three inoculation methods on determination of antibacterial activity:
a) absorption method (an evaluation method in which the test bacterial suspension is inoculated directly onto specimens);
b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens);
c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens).
The colony plate count method and the ATP (ATP = Adenosine Tri-phosphate) luminescence method are also specified for measuring the enumeration of bacteria.
<p>ISO 20743:2007 specifies quantitative test methods to determine the antibacterial activity of antibacterial finished textile products including nonwovens.</p> <p>ISO 20743:2007 is applicable to all textile products, including cloth, wadding, thread and material for clothing, home furnishings and miscellaneous goods regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).</p> <p>Based on the intended application and on the environment in which the textile product is to be used, the user can select the most suitable of the following three methods on determination of antibacterial activity: a) absorption method (an evaluation method in which test bacterial suspension is inoculated directly onto samples); b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto samples); c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto samples). </p> <p>The colony plate count method and the ATP (Adenosine Tri-phosphate) luminescence method are also specified for measuring the enumeration of bacteria.</p>
<p>This document specifies quantitative test methods to determine the antibacterial activity of all antibacterial textile products including nonwovens.</p> <p>This document is applicable to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnishings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).</p> <p>This document covers three inoculation methods for the determination of antibacterial activity:</p> <p>a) absorption method (an evaluation method in which the test bacterial suspension is inoculated directly onto specimens);</p> <p>b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens);</p> <p>c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens).</p> <p>NOTE Based on the intended application and on the environment in which the textile product is to be used, and also on the surface properties of the textile properties, the user can select the most suitable inoculation method.</p> <p>This document also specifies the colony plate count method and the adenosine triphosphate (ATP) luminescence method for measuring the enumeration of bacteria.</p>
- Avant-proposiv
- Introductionv
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1 Domaine d'application1
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2 Références normatives1
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3 Termes et définitions1
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4 Mesures de sécurité2
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5 Appareillage2
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6 Réactifs et milieux de culture3
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6.1 Eau4
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6.2 Bouillon tryptone soja (TSB)4
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6.3 Gélose tryptone soja (TSA)4
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6.4 Gélose pour transfert4
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6.5 Bouillon nutritif5
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6.6 Eau peptonée saline5
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6.7 Solution physiologique saline5
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6.8 Milieu de culture SCDLP5
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6.9 Tampon de dilution pour la suspension bactérienne d'extraction6
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6.10 Solution neutralisante6
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6.11 Gélose de dénombrement6
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6.12 Gélose pour impression6
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6.13 Solution cryoprotectrice pour espèces bactériennes7
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6.14 Solution mère du réactif standard d'ATP7
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6.15 Solution tampon pour le réactif luminescent à l'ATP7
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6.16 Réactif luminescent à l'ATP8
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6.17 Réactif d'extraction de l'ATP8
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6.18 Réactif d'élimination de l'ATP8
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6.19 Milieu SCDLP ou autre milieu pour la préparation de la solution de référence d'ATP8
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6.20 Solution physiologique saline d'extraction9
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7 Souches de référence9
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7.1 Souches9
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7.2 Conservation des souches9
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8 Modes opératoires d'essai10
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8.1 Méthode par absorption (voir Annexe E)10
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8.2 Méthode par transfert (voir Annexe E)15
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8.3 Méthode par impression (voir Annexe E)19
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9 Rapport d'essai23
- Annexe A (normative) Numéros de souches24
- Annexe B (normative) Méthode d'agitation25
- Annexe C (normative) Mesurage quantitatif avec la méthode par dénombrement26
- Annexe D (normative) Mesurage quantitatif avec la méthode par luminescence27
- Annexe E (informative) Exemples d'essais29
- Annexe F (informative) Efficacité de l'activité antibactérienne33
- Bibliographie34
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