NF EN ISO 20743

NF EN ISO 20743

September 2013
Standard Cancelled

Textiles - Determination of antibacterial activity of textile products

ISO 20743:2013 specifies quantitative test methods to determine the antibacterial activity of all antibacterial textile products including nonwovens. ISO 20743:2013 is applicable to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnishings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting). Based on the intended application and on the environment in which the textile product is to be used and also on the surface properties of the textile properties, the user can select the most suitable of the following three inoculation methods on determination of antibacterial activity: a) absorption method (an evaluation method in which the test bacterial suspension is inoculated directly onto specimens); b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens); c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens). The colony plate count method and the ATP (ATP = Adenosine Tri-phosphate) luminescence method are also specified for measuring the enumeration of bacteria.

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Main informations

Collections

National standards and national normative documents

Publication date

September 2013

Number of pages

44 p.

Reference

NF EN ISO 20743

ICS Codes

07.100.99   Other standards related to microbiology
59.080.01   Textiles in general

Classification index

G39-020

Print number

1 - 06/09/2013

International kinship

European kinship

EN ISO 20743:2013
Sumary
Textiles - Determination of antibacterial activity of textile products

ISO 20743:2013 specifies quantitative test methods to determine the antibacterial activity of all antibacterial textile products including nonwovens.

ISO 20743:2013 is applicable to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnishings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).

Based on the intended application and on the environment in which the textile product is to be used and also on the surface properties of the textile properties, the user can select the most suitable of the following three inoculation methods on determination of antibacterial activity:

a) absorption method (an evaluation method in which the test bacterial suspension is inoculated directly onto specimens);

b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens);

c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens).

The colony plate count method and the ATP (ATP = Adenosine Tri-phosphate) luminescence method are also specified for measuring the enumeration of bacteria.

Replaced standards (1)
NF EN ISO 20743
September 2007
Standard Cancelled
Textiles - Determination of antibacterial activity of antibacterial finished products

<p>ISO 20743:2007 specifies quantitative test methods to determine the antibacterial activity of antibacterial finished textile products including nonwovens.</p> <p>ISO 20743:2007 is applicable to all textile products, including cloth, wadding, thread and material for clothing, home furnishings and miscellaneous goods regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).</p> <p>Based on the intended application and on the environment in which the textile product is to be used, the user can select the most suitable of the following three methods on determination of antibacterial activity: a) absorption method (an evaluation method in which test bacterial suspension is inoculated directly onto samples); b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto samples); c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto samples). </p> <p>The colony plate count method and the ATP (Adenosine Tri-phosphate) luminescence method are also specified for measuring the enumeration of bacteria.</p>

Standard replaced by (1)
NF EN ISO 20743
June 2021
Standard Current
Textiles - Determination of antibacterial activity of textile products

<p>This document specifies quantitative test methods to determine the antibacterial activity of all antibacterial textile products including nonwovens.</p> <p>This document is applicable to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnishings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).</p> <p>This document covers three inoculation methods for the determination of antibacterial activity:</p> <p>a) absorption method (an evaluation method in which the test bacterial suspension is inoculated directly onto specimens);</p> <p>b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens);</p> <p>c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens).</p> <p>NOTE            Based on the intended application and on the environment in which the textile product is to be used, and also on the surface properties of the textile properties, the user can select the most suitable inoculation method.</p> <p>This document also specifies the colony plate count method and the adenosine triphosphate (ATP) luminescence method for measuring the enumeration of bacteria.</p>

Table of contents
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  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    1
  • 4 Mesures de sécurité
    2
  • 5 Appareillage
    2
  • 6 Réactifs et milieux de culture
    3
  • 6.1 Eau
    4
  • 6.2 Bouillon tryptone soja (TSB)
    4
  • 6.3 Gélose tryptone soja (TSA)
    4
  • 6.4 Gélose pour transfert
    4
  • 6.5 Bouillon nutritif
    5
  • 6.6 Eau peptonée saline
    5
  • 6.7 Solution physiologique saline
    5
  • 6.8 Milieu de culture SCDLP
    5
  • 6.9 Tampon de dilution pour la suspension bactérienne d'extraction
    6
  • 6.10 Solution neutralisante
    6
  • 6.11 Gélose de dénombrement
    6
  • 6.12 Gélose pour impression
    6
  • 6.13 Solution cryoprotectrice pour espèces bactériennes
    7
  • 6.14 Solution mère du réactif standard d'ATP
    7
  • 6.15 Solution tampon pour le réactif luminescent à l'ATP
    7
  • 6.16 Réactif luminescent à l'ATP
    8
  • 6.17 Réactif d'extraction de l'ATP
    8
  • 6.18 Réactif d'élimination de l'ATP
    8
  • 6.19 Milieu SCDLP ou autre milieu pour la préparation de la solution de référence d'ATP
    8
  • 6.20 Solution physiologique saline d'extraction
    9
  • 7 Souches de référence
    9
  • 7.1 Souches
    9
  • 7.2 Conservation des souches
    9
  • 8 Modes opératoires d'essai
    10
  • 8.1 Méthode par absorption (voir Annexe E)
    10
  • 8.2 Méthode par transfert (voir Annexe E)
    15
  • 8.3 Méthode par impression (voir Annexe E)
    19
  • 9 Rapport d'essai
    23
  • Annexe A (normative) Numéros de souches
    24
  • Annexe B (normative) Méthode d'agitation
    25
  • Annexe C (normative) Mesurage quantitatif avec la méthode par dénombrement
    26
  • Annexe D (normative) Mesurage quantitatif avec la méthode par luminescence
    27
  • Annexe E (informative) Exemples d'essais
    29
  • Annexe F (informative) Efficacité de l'activité antibactérienne
    33
  • Bibliographie
    34
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