NF EN ISO 20836

NF EN ISO 20836

December 2021
Standard Current

Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of microorganisms - Thermal performance testing of thermal cyclers

This document specifies requirements for the installation, maintenance, temperature calibration and temperature performance testing of standard thermal cyclers and real-time thermal cyclers. It is applicable to the detection of microorganisms as well as any other applications in the food chain using polymerase chain reaction (PCR)-based methods. This document has been established for food testing, but is also applicable to other domains using thermal cyclers (e.g. environmental, human health, animal health, forensic testing).

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Main informations

Collections

National standards and national normative documents

Publication date

December 2021

Number of pages

36 p.

Reference

NF EN ISO 20836

ICS Codes

07.100.30   Food microbiology

Classification index

V03-027

Print number

1

International kinship

ISO 20836:2021

European kinship

EN ISO 20836:2021
Sumary
Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of microorganisms - Thermal performance testing of thermal cyclers

This document specifies requirements for the installation, maintenance, temperature calibration and temperature performance testing of standard thermal cyclers and real-time thermal cyclers. It is applicable to the detection of microorganisms as well as any other applications in the food chain using polymerase chain reaction (PCR)-based methods.

This document has been established for food testing, but is also applicable to other domains using thermal cyclers (e.g. environmental, human health, animal health, forensic testing).

Replaced standards (1)
XP CEN ISO/TS 20836
May 2007
Standard Cancelled
Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Performance testing for thermal cyclers

Le présent document précise des exigences fondamentales concernant l'installation, la performance et la maintenance des thermocycleurs. Bien que ces équipements soient des dispositifs solides, ils nécessitent une maintenance régulière. Leurs éléments chauffants/refroidissants à effet Peltier ou équivalent, ont une durée de vie limitée. Le bon fonctionnement de l'élément chauffant/refroidissant dépend à la fois de la qualité des dispositifs de chauffage/refroidissement et d'une utilisation et de maintenances appropriées. En plus de la définition des exigences pour un programme de maintenance défini, sont décrits les modes opératoires pour la détermination de la performance des thermocycleurs par des méthodes biochimiques ou physiques (voir les Annexes A et B).

Table of contents
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  • Avant-propos
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Installation des thermocycleurs
  • 5 Maintenance des thermocycleurs
  • 6 Essai de performance des thermocycleurs
  • Annexe A (informative) Emplacement des sondes
  • Annexe B (informative) Protocole de température universel
  • Annexe C (informative) Essai de conformité
  • Annexe D (informative) Exemple de profil thermique d'un thermocycleur
  • Annexe E (informative) Exemple d'essai de performance et d'essai de conformité
  • Bibliographie
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