NF EN ISO 21150

NF EN ISO 21150

September 2009
Standard Cancelled

Cosmetics - Microbiology - Detection of Escherichia coli

ISO 21150:2006 gives general guidelines for the detection and identification of the specified microorganism Escherichia coli in cosmetic products. Microorganisms considered as specified in ISO 21150:2006 might differ from country to country according to national practices or regulations. ISO 21150:2006 specifies a method that is based on the detection of Escherichia coli in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate depending on the level of detection required. Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water-immiscible products). Other International Standards may be more appropriate.

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Main informations

Collections

National standards and national normative documents

Publication date

September 2009

Number of pages

23 p.

Reference

NF EN ISO 21150

ICS Codes

07.100.40   Cosmetics microbiology

Classification index

T75-604

Print number

1 - 01/09/2009

International kinship

European kinship

EN ISO 21150:2009
Sumary
Cosmetics - Microbiology - Detection of Escherichia coli

ISO 21150:2006 gives general guidelines for the detection and identification of the specified microorganism Escherichia coli in cosmetic products. Microorganisms considered as specified in ISO 21150:2006 might differ from country to country according to national practices or regulations.

ISO 21150:2006 specifies a method that is based on the detection of Escherichia coli in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate depending on the level of detection required.

Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water-immiscible products). Other International Standards may be more appropriate.

Replaced standards (1)
NF ISO 21150
September 2006
Standard Cancelled
Cosmetics - Microbiology - Detection of Escherichia coli

<p>ISO 21150:2006 gives general guidelines for the detection and identification of the specified microorganism <i>Escherichia coli</i> in cosmetic products. Microorganisms considered as specified in ISO 21150:2006 might differ from country to country according to national practices or regulations.</p> <p>ISO 21150:2006 specifies a method that is based on the detection of <i>Escherichia coli</i> in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate depending on the level of detection required.</p> <p>Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water-immiscible products). Other International Standards may be more appropriate. </p>

Standard replaced by (1)
NF EN ISO 21150
February 2016
Standard Current
Cosmetics - Microbiology - Detection of Escherichia coli

<p>ISO 21150:2015 gives general guidelines for the detection and identification of the specified microorganism Escherichia coli in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations.</p> <p>In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis, so as to determine the types of cosmetic products to which this International Standard is applicable. Products considered to present a low microbiological (see ISO 29621) risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc.</p> <p>The method described in this International Standard is based on the detection of Escherichia coli in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate, depending on the level of detection required.</p> <p>NOTE For the detection of Escherichia coli, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits).</p> <p>Because of the large variety of cosmetic products within this field of application, this method might not be suited to some products in every detail (e.g. certain water-immiscible products). Other International Standards (ISO 18415) may be appropriate. Other methods (e.g. automated) can be substituted for the test presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.</p>

Table of contents
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  • Avant-propos
    iv
  • Introduction
    v
  • 1 Domaine d'application
    1
  • 2 Références normatives
    1
  • 3 Termes et définitions
    1
  • 4 Principe
    2
  • 5 Diluants et milieux de culture
    2
  • 5.1 Généralités
    2
  • 5.2 Diluant pour la suspension bactérienne (Solution tryptone-sel)
    3
  • 5.3 Milieux de culture
    3
  • 6 Appareillage et verrerie
    6
  • 7 Souches de micro-organismes
    6
  • 8 Manipulation des produits cosmétiques et des échantillons de laboratoire
    6
  • 9 Mode opératoire
    6
  • 9.1 Recommandations générales
    6
  • 9.2 Préparation de la suspension initiale dans le milieu liquide d'enrichissement
    7
  • 9.3 Incubation du milieu liquide d'enrichissement ensemencé
    7
  • 9.4 Recherche et identification d'Escherichia coli
    7
  • 10 Expression des résultats (recherche d'Escherichia coli)
    8
  • 11 Neutralisation des propriétés antimicrobiennes du produit
    8
  • 11.1 Généralités
    8
  • 11.2 Préparation de l'inoculum
    9
  • 11.3 Validation de la méthode de recherche
    9
  • 12 Rapport d'essai
    10
  • Annexe A (informative) Autres milieux liquides d'enrichissement
    11
  • Annexe B (informative) Neutralisants de l'activité antimicrobienne des conservateurs et liquides de rinçage
    14
  • Bibliographie
    15
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