NF EN ISO 21528-1

NF EN ISO 21528-1

July 2017
Standard Current

Microbiology of the food chain - Horizontal method for the detection and enumeration of Enterobacteriaceae - Part 1 : Detection of Enterobacteriaceae

ISO 21528-1:2017 specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to- products intended for human consumption and the feeding of animals, and- environmental samples in the area of primary production, food production and food handling.This method is applicable- when the microorganisms sought are expected to need resuscitation by enrichment, and- when the number sought is expected to be below 100 per millilitre or per gram of test sample.A limitation on the applicability of ISO 21528-1:2017 is imposed by the susceptibility of the method to a large degree of variability (see Clause 11).NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See Annex A.

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Main informations

Collections

National standards and national normative documents

Publication date

July 2017

Number of pages

27 p.

Reference

NF EN ISO 21528-1

ICS Codes

07.100.30   Food microbiology

Classification index

V08-039-1

Print number

1

International kinship

ISO 21528-1:2017

European kinship

EN ISO 21528-1:2017
Sumary
Microbiology of the food chain - Horizontal method for the detection and enumeration of Enterobacteriaceae - Part 1 : Detection of Enterobacteriaceae

ISO 21528-1:2017 specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to

- products intended for human consumption and the feeding of animals, and

- environmental samples in the area of primary production, food production and food handling.

This method is applicable

- when the microorganisms sought are expected to need resuscitation by enrichment, and

- when the number sought is expected to be below 100 per millilitre or per gram of test sample.

A limitation on the applicability of ISO 21528-1:2017 is imposed by the susceptibility of the method to a large degree of variability (see Clause 11).

NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See Annex A.

Replaced standards (1)
NF ISO 21528-1
December 2004
Standard Cancelled
Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 1 : detection and enumeration by MPN technique with pre-enrichment

Le présent document décrit une méthode pour la recherche des Enterobacteriaceae avec pré-enrichissement applicable aux produits pour l'alimentation humaine et animale, échantillons d'environnement pour la production et la distribution des aliments.

Table of contents
  • Avant-propos
  • 1
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Diluants, milieux de culture et réactifs
  • 6 Matériel et consommables
  • 7 Échantillonnage
  • 8 Préparation de l'échantillon pour essai
  • 9 Mode opératoire
  • 10 Expression des résultats
  • 11 Fidélité
  • 12 Rapport d'essai
  • 13 Assurance qualité
  • Annexe B (normative) Milieux de culture et réactifs
  • Annexe A (informative) Dénombrement à l'aide de la technique NPP
  • Annexe C (informative) Études de validation de la méthode et caractéristiques de performance
  • Bibliographie
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