NF EN ISO 21528-1
Microbiology of the food chain - Horizontal method for the detection and enumeration of Enterobacteriaceae - Part 1 : Detection of Enterobacteriaceae
ISO 21528-1:2017 specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to- products intended for human consumption and the feeding of animals, and- environmental samples in the area of primary production, food production and food handling.This method is applicable- when the microorganisms sought are expected to need resuscitation by enrichment, and- when the number sought is expected to be below 100 per millilitre or per gram of test sample.A limitation on the applicability of ISO 21528-1:2017 is imposed by the susceptibility of the method to a large degree of variability (see Clause 11).NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See Annex A.
ISO 21528-1:2017 specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to
- products intended for human consumption and the feeding of animals, and
- environmental samples in the area of primary production, food production and food handling.
This method is applicable
- when the microorganisms sought are expected to need resuscitation by enrichment, and
- when the number sought is expected to be below 100 per millilitre or per gram of test sample.
A limitation on the applicability of ISO 21528-1:2017 is imposed by the susceptibility of the method to a large degree of variability (see Clause 11).
NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See Annex A.
ISO 21528-1:2004 specifies a method, with pre-enrichment, for the detection of Enterobacteriaceae. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling. Enumeration is carried out by calculation of the most probable number (MPN) after incubation at 37 °C in liquid medium. This method is applicable when the microorganisms sought are expected to need resuscitation before enrichment, and when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample. A limitation on the applicability of ISO 21528-1:2004 is imposed by the susceptibility of the method to a large degree of variability
- Avant-propos
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1
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2 Références normatives
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3 Termes et définitions
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4 Principe
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5 Diluants, milieux de culture et réactifs
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6 Matériel et consommables
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7 Échantillonnage
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8 Préparation de l'échantillon pour essai
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9 Mode opératoire
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10 Expression des résultats
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11 Fidélité
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12 Rapport d'essai
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13 Assurance qualité
- Annexe B (normative) Milieux de culture et réactifs
- Annexe A (informative) Dénombrement à l'aide de la technique NPP
- Annexe C (informative) Études de validation de la méthode et caractéristiques de performance
- Bibliographie
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